Literature DB >> 20516604

Expression, purification and crystallization of human prolylcarboxypeptidase.

Pravien D Abeywickrema1, Sangita B Patel, Noel J Byrne, Ronald E Diehl, Dawn L Hall, Rachael E Ford, Keith W Rickert, John C Reid, Jennifer M Shipman, Wayne M Geissler, Kelly D Pryor, Ranabir SinhaRoy, Stephen M Soisson, Kevin J Lumb, Sujata Sharma.   

Abstract

Prolylcarboxypeptidase (PrCP) is a lysosomal serine carboxypeptidase that cleaves a variety of C-terminal amino acids adjacent to proline and has been implicated in diseases such as hypertension and obesity. Here, the robust production, purification and crystallization of glycosylated human PrCP from stably transformed CHO cells is described. Purified PrCP yielded crystals belonging to space group R32, with unit-cell parameters a = b = 181.14, c = 240.13 A, that diffracted to better than 2.8 A resolution.

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Year:  2010        PMID： 20516604      PMCID： PMC2882774          DOI： 10.1107/S1744309110014041

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  9 in total

1.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

2.  Purification and properties of prolylcarboxypeptidase (angiotensinase C) from human kidney.

Authors:  C E Odya; D V Marinkovic; K J Hammon; T A Stewart; E G Erdös
Journal:  J Biol Chem       Date:  1978-09-10       Impact factor: 5.157

3.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

4.  Identification and characterization of prolylcarboxypeptidase as an endothelial cell prekallikrein activator.

Authors:  Zia Shariat-Madar; Fakhri Mahdi; Alvin H Schmaier
Journal:  J Biol Chem       Date:  2002-02-05       Impact factor: 5.157

5.  Prolylcarboxypeptidase regulates food intake by inactivating alpha-MSH in rodents.

Authors:  Nicholas Wallingford; Bertrand Perroud; Qian Gao; Anna Coppola; Erika Gyengesi; Zhong-Wu Liu; Xiao-Bing Gao; Adam Diament; Kari A Haus; Zia Shariat-Madar; Fakhri Mahdi; Sharon L Wardlaw; Alvin H Schmaier; Craig H Warden; Sabrina Diano
Journal:  J Clin Invest       Date:  2009-07-20       Impact factor: 14.808

6.  Structural basis of human p70 ribosomal S6 kinase-1 regulation by activation loop phosphorylation.

Authors:  Tomoko Sunami; Noel Byrne; Ronald E Diehl; Kaoru Funabashi; Dawn L Hall; Mari Ikuta; Sangita B Patel; Jennifer M Shipman; Robert F Smith; Ikuko Takahashi; Joan Zugay-Murphy; Yoshikazu Iwasawa; Kevin J Lumb; Sanjeev K Munshi; Sujata Sharma
Journal:  J Biol Chem       Date:  2009-10-28       Impact factor: 5.157

7.  Sequencing and cloning of human prolylcarboxypeptidase (angiotensinase C). Similarity to both serine carboxypeptidase and prolylendopeptidase families.

Authors:  F Tan; P W Morris; R A Skidgel; E G Erdös
Journal:  J Biol Chem       Date:  1993-08-05       Impact factor: 5.157

8.  Recombinant prolylcarboxypeptidase activates plasma prekallikrein.

Authors:  Zia Shariat-Madar; Fakhri Mahdi; Alvin H Schmaier
Journal:  Blood       Date:  2004-03-02       Impact factor: 22.113

Review 9.  Prolylcarboxypeptidase: a cardioprotective enzyme.

Authors:  J Mallela; J Yang; Z Shariat-Madar
Journal:  Int J Biochem Cell Biol       Date:  2008-02-29       Impact factor: 5.085
  9 in total
  5 in total

1.  Ghrelin regulates hypothalamic prolyl carboxypeptidase expression in mice.

Authors:  Jin Kwon Jeong; Jung Dae Kim; Sabrina Diano
Journal:  Mol Metab       Date:  2013-01-11       Impact factor: 7.422

2.  Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis.

Authors:  Gregory N Adams; Gretchen A LaRusch; Evi Stavrou; Yihua Zhou; Marvin T Nieman; Gretta H Jacobs; Yingjie Cui; Yuan Lu; Mukesh K Jain; Fakhri Mahdi; Zia Shariat-Madar; Yoshio Okada; Louis G D'Alecy; Alvin H Schmaier
Journal:  Blood       Date:  2011-02-04       Impact factor: 22.113

3.  Prolyl carboxypeptidase and its inhibitors in metabolism.

Authors:  Jin Kwon Jeong; Sabrina Diano
Journal:  Trends Endocrinol Metab       Date:  2012-12-12       Impact factor: 12.015

4.  Structural definition and substrate specificity of the S28 protease family: the crystal structure of human prolylcarboxypeptidase.

Authors:  Stephen M Soisson; Sangita B Patel; Pravien D Abeywickrema; Noel J Byrne; Ronald E Diehl; Dawn L Hall; Rachael E Ford; John C Reid; Keith W Rickert; Jennifer M Shipman; Sujata Sharma; Kevin J Lumb
Journal:  BMC Struct Biol       Date:  2010-06-11

5.  Prolyl carboxypeptidase mRNA expression in the mouse brain.

Authors:  Jin Kwon Jeong; Sabrina Diano
Journal:  Brain Res       Date:  2013-10-24       Impact factor: 3.252
  5 in total

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