| Literature DB >> 20516592 |
A V Lyashenko1, E Y Bezsudnova, V M Gumerov, A A Lashkov, A V Mardanov, A M Mikhailov, K M Polyakov, V O Popov, N V Ravin, K G Skryabin, V K Zabolotniy, T N Stekhanova, M V Kovalchuk.
Abstract
Alcohol dehydrogenases belong to the oxidoreductase family and play an important role in a broad range of physiological processes. They catalyze the cofactor-dependent reversible oxidation of alcohols to the corresponding aldehydes or ketones. The NADP-dependent short-chain alcohol dehydrogenase TsAdh319 from the thermophilic archaeon Thermococcus sibiricus was overexpressed, purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion method using 25%(w/v) polyethylene glycol 3350 pH 7.5 as precipitant. The crystals diffracted to 1.68 A resolution and belonged to space group I222, with unit-cell parameters a = 55.63, b = 83.25, c = 120.75 A.Entities:
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Year: 2010 PMID： 20516592 PMCID： PMC2882762 DOI： 10.1107/S1744309110002654
Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091