Literature DB >> 20516190

Determining the yield and quality of purified RNA.

Donald C Rio, Manuel Ares, Gregory J Hannon, Timothy W Nilsen.   

Abstract

After any RNA purification by any method, it is necessary to ascertain both the amount (yield) and the integrity (quality) of the RNA obtained. This is essential for any subsequent analysis and critical for any comparative analyses. This article discusses several methods for RNA quantitation and provides tips for each.

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Year:  2010        PMID: 20516190     DOI: 10.1101/pdb.top82

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  2 in total

1.  Molecular cloning, expression, and in silico structural analysis of guinea pig IL-17.

Authors:  Vijaya R Dirisala; Amminikutty Jeevan; Suresh K Ramasamy; David N McMurray
Journal:  Mol Biotechnol       Date:  2013-11       Impact factor: 2.695

2.  Assessing the quality of RNA isolated from human breast tissue after ambient room temperature exposure.

Authors:  Stella B Somiari; Susan Shuss; Jianfang Liu; Kimberly Mamula; Amy O'Donnell; Brenda Deyarmin; Jennifer Kane; Amber Greenawalt; Caroline Larson; Sean Rigby; Hai Hu; Craig D Shriver
Journal:  PLoS One       Date:  2022-01-18       Impact factor: 3.240

  2 in total

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