Antidiabetic effects of Eucalyptus globulus on pancreatic islets: a stereological study.

The leaves of Eucalyptus globulus (eucalyptus) are used for the treatment of diabetes mellitus in traditional medicine. The aim of this study was to evaluate the effects of eucalyptus on streptozotocin (STZ)-induced damage in pancreatic islands by stereological methods. Fifty mature normoglycaemic male Wistar rats, weighing 200-250 g, were selected and randomly divided into 5 groups (n = 10): control; STZ-induced diabetic (D) - by intraperitoneal injection of 60 mg/kg streptozotocin; treated control (TC); and treated diabetic (TD1, 2), respectively, received 20 and 62.5 g/kg of eucalyptus in their diet, and 2.5 g/L aqueous extract of eucalyptus in their drinking water from one week after induction of diabetes. After four weeks of the experiment, stereological estimation of volume density and total volume of islets and beta cells, volume-weighted mean islet volume, mass of the islets and pancreas, and total number of islets were carried out. Administration of eucalyptus significantly decreased the weight loss and increase of water and food intake in the treated diabetic groups in comparison to the STZ-induced diabetic (D) group. Volume density and total volume of islets, volume-weighted mean islet volume, mass of islets, and mass of pancreas of both treated diabetic groups were higher than the D group. In TD2, these stereological parameters increased significantly compared to the D group (p < 0.001). Volume density and total volume of beta cells increased 21% and 65%, respectively, in the TD2 group, but it was not statistically significant compared to the diabetic group (p > 0.05). The results suggested that Eucalyptus globulus with a dose-dependent manner ameliorates diabetic states by partial restoration of pancreatic beta cells and repair of STZ-induced damage in rats. This study suggests a beneficial effect of eucalyptus in the treatment of diabetes.