Literature DB >> 20512626

Swine virus-specific cytotoxic cells producing IFNgamma under different conditions of virus antigenic stimulation.

L Ferrari1, E De Angelis, P Martelli, P Borghetti.   

Abstract

Flow cytometry is a useful tool to determine both the phenotype and function of immune cells during vaccination and viral infection. Since cytokine release is a hallmark of cell activation, detection of intracellular interferon (IFN)gamma in cytotoxic CD8alpha+ cells under physiological conditions and after Aujeszky's Disease Virus (ADV)- or Porcine Circovirus type 2 (PCV2)-swine interaction was carried out. Blood samples were collected from healthy 10-month-old pigs, ADV- or PCV2-vaccinated pigs, and PCV2-infected pigs; the levels of total IFNgamma+ and CD8alpha+IFNgamma+ cells were evaluated after PMA-ionomycin and virus-specific in vitro stimulation. High CD8alpha+IFNgamma+ cell levels were detected in adult pigs, whereas lower virus-specific cell fractions were observed after ADV or PCV2 vaccination as well as after PCV2 natural infection due to restricted activation. Such results support the use of cytokine intracellular staining to monitor virus-specific cell-mediated immunity.

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Year:  2010        PMID： 20512626     DOI： 10.1007/s11259-010-9383-4

Source DB:  PubMed          Journal:  Vet Res Commun        ISSN： 0165-7380            Impact factor:   2.459


  6 in total

1.  Phenotypic characterization of porcine IFN-gamma-producing lymphocytes by flow cytometry.

Authors:  M P Rodríguez-Carreño; L López-Fuertes; C Revilla; A Ezquerra; F Alonso; J Domínguez
Journal:  J Immunol Methods       Date:  2002-01-01       Impact factor: 2.303

2.  Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1.

Authors:  R Paillot; J M Daly; V Juillard; J M Minke; D Hannant; J H Kydd
Journal:  Vaccine       Date:  2005-08-22       Impact factor: 3.641

3.  Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection.

Authors:  W Sipos; J C Duvigneau; M Willheim; F Schilcher; R T Hartl; G Hofbauer; B Exel; P Pietschmann; F Schmoll
Journal:  Vet Immunol Immunopathol       Date:  2004-05       Impact factor: 2.046

4.  Time course of the porcine cellular and humoral immune responses in vivo against pseudorabies virus after inoculation and challenge: significance of in vitro antigenic restimulation.

Authors:  M G De Bruin; Y E De Visser; T G Kimman; A T Bianchi
Journal:  Vet Immunol Immunopathol       Date:  1998-09-16       Impact factor: 2.046

5.  Porcine circovirus type 2 (PCV2) viral components immunomodulate recall antigen responses.

Authors:  Tuija Kekarainen; Maria Montoya; Javier Dominguez; Enric Mateu; Joaquim Segalés
Journal:  Vet Immunol Immunopathol       Date:  2008-01-21       Impact factor: 2.046

6.  Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens.

Authors:  Laila Darwich; Mònica Balasch; Joan Plana-Durán; Joaquim Segalés; Mariano Domingo; Enric Mateu
Journal:  J Gen Virol       Date:  2003-12       Impact factor: 3.891
  6 in total
  1 in total

1.  Memory T cell proliferative responses and IFN-γ productivity sustain long-lasting efficacy of a Cap-based PCV2 vaccine upon PCV2 natural infection and associated disease.

Authors:  Luca Ferrari; Paolo Borghetti; Elena De Angelis; Paolo Martelli
Journal:  Vet Res       Date:  2014-04-16       Impact factor: 3.683
  1 in total

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