PURPOSE: Helicobacter pylori (H. pylori) is considered to be a major factor contributing to gastric mucosal damage by stimulating mucosal macrophage production of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), but the inflammatory responses within the gastric mucosa in vivo are not well known. Therefore, this study was designed to investigate the expression of TNF-α induced by H. pylori infection which is involved in the tumor initiation and promotion of gastric carcinogenesis. METHODS: This study was carried out in 200 patients, consisting of normal gastric mucosa (n = 20), mucosa with chronic gastritis (n = 63), intestinal metaplasia (n = 20), dysplasia (n = 11), and gastric adenocarcinoma (n = 86), in which the H. pylori status has been analyzed. The expression of TNF-α was studied at mRNA as well as protein level using RT-PCR and western blotting, respectively. The localization of TNF-α was also studied semiquantitatively by immunohistochemistry. RESULTS: The RT-PCR and western blotting results of TNF-α mRNA and protein expressions were significantly increased in chronic gastritis, intestinal metaplasia, dysplasia and gastric adenocarcinoma patients, respectively. Immunohistochemical study also showed the increased expression of TNF-α in the similar way. CONCLUSION: Over expression of TNF-α showed a significant severity-dose-response as risk markers from preneoplastic lesions to gastric cancer.
PURPOSE:Helicobacter pylori (H. pylori) is considered to be a major factor contributing to gastric mucosal damage by stimulating mucosal macrophage production of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), but the inflammatory responses within the gastric mucosa in vivo are not well known. Therefore, this study was designed to investigate the expression of TNF-α induced by H. pyloriinfection which is involved in the tumor initiation and promotion of gastric carcinogenesis. METHODS: This study was carried out in 200 patients, consisting of normal gastric mucosa (n = 20), mucosa with chronic gastritis (n = 63), intestinal metaplasia (n = 20), dysplasia (n = 11), and gastric adenocarcinoma (n = 86), in which the H. pylori status has been analyzed. The expression of TNF-α was studied at mRNA as well as protein level using RT-PCR and western blotting, respectively. The localization of TNF-α was also studied semiquantitatively by immunohistochemistry. RESULTS: The RT-PCR and western blotting results of TNF-α mRNA and protein expressions were significantly increased in chronic gastritis, intestinal metaplasia, dysplasia and gastric adenocarcinomapatients, respectively. Immunohistochemical study also showed the increased expression of TNF-α in the similar way. CONCLUSION: Over expression of TNF-α showed a significant severity-dose-response as risk markers from preneoplastic lesions to gastric cancer.
Authors: R A Hatz; G Rieder; M Stolte; E Bayerdörffer; G Meimarakis; F W Schildberg; G Enders Journal: Gastroenterology Date: 1997-06 Impact factor: 22.682
Authors: J Rudi; D Kuck; S Strand; A von Herbay; S M Mariani; P H Krammer; P R Galle; W Stremmel Journal: J Clin Invest Date: 1998-10-15 Impact factor: 14.808
Authors: B K Erikstein; E B Smeland; H K Blomhoff; S Funderud; K Prydz; W Lesslauer; T Espevik Journal: Eur J Immunol Date: 1991-04 Impact factor: 5.532
Authors: Tilman T Rau; Anja Rogler; Myrjam Frischauf; Andreas Jung; Peter C Konturek; Arno Dimmler; Gerhard Faller; Bettina Sehnert; Wael El-Rifai; Arndt Hartmann; Reinhard E Voll; Regine Schneider-Stock Journal: Am J Pathol Date: 2012-06-27 Impact factor: 4.307