| Literature DB >> 20510876 |
Yanping Tian1, Wen Jiang, Na Gao, Junlei Zhang, Wei Chen, Dongying Fan, Deshan Zhou, Jing An.
Abstract
Reduced glutathione (GSH) is the most powerful intracellular antioxidant and also involved in viral infections. The pathogenesis of dengue virus (DV) infection has not been completely clarified. This study investigated the relationship between DV serotype 2 (DV2) infections and host intracellular GSH content. Results showed infection with DV2 resulted in a decrease in intracellular GSH, which caused NF-kappaB activation and increased DV2 production. Supplemental GSH significantly inhibited activation of NF-kappaB, resulting in a decreased production of DV2 in HepG2 cells. Furthermore, high activity of NF-kappaB and increased production of DV2 was observed in HepG2 cells treated with buthionine sulfoximine (BSO), an inhibitor of GSH synthesis. In conclusion, DV2 infection could reduce host intracellular GSH concentration and benefited from this process. Supplemental GSH could inhibit viral production, indicating GSH might be valuable in the prevention and treatment of DV2 infection. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20510876 DOI： 10.1016/j.bbrc.2010.05.108
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575