Literature DB >> 2051018

Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies.

T Saito1, K Yasukawa, H Suzuki, K Futatsugi, T Fukunaga, C Yokomizo, Y Koishihara, H Fukui, Y Ohsugi, H Yawata.   

Abstract

Starting with a previously isolated cDNA clone encoding murine IL-6R, a stable transformed Chinese hamster ovary cell line constitutively expressing soluble murine IL-6R (smIL-6R) has been established. The smIL-6R was purified to homogeneity by sequential filtration and chromatography of culture medium. The smIL-6R augmented the sensitivity of M1 cells to IL-6 in their growth inhibition in a dose-response manner. Rat hybridomas producing mAb specific to murine IL-6R were also established. One of the clones, RS13, produced IgG2a isotype that was capable of inhibiting IL-6 activity. ELISA for the quantitation of smIL-6R was established, which could detect smIL-6R in a quantity as low as 1 ng/ml.

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Year:  1991        PMID： 2051018

Source DB:  PubMed          Journal:  J Immunol        ISSN： 0022-1767            Impact factor:   5.422


  11 in total

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Journal:  Neurobiol Aging       Date:  2000 May-Jun       Impact factor: 4.673

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6.  Soluble interleukin-6 receptor triggers osteoclast formation by interleukin 6.

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10.  Interleukin (IL)-6 induction of osteoclast differentiation depends on IL-6 receptors expressed on osteoblastic cells but not on osteoclast progenitors.

Authors:  N Udagawa; N Takahashi; T Katagiri; T Tamura; S Wada; D M Findlay; T J Martin; H Hirota; T Taga; T Kishimoto; T Suda
Journal:  J Exp Med       Date:  1995-11-01       Impact factor: 14.307
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