Literature DB >> 20496113

CD40 expression in Wehi-164 cell line.

Mohammad Hossein Karimi1, Padideh Ebadi, Ali Akbar Pourfathollah, Zahra Soheila Soheili, Seyed Mohammad Moazzeni.   

Abstract

CD40-CD154 interaction is an important process for cellular and humoral immunity regulation and can be effective in the body's defense against tumors. In the present study, we evaluated the expression of CD40 in Wehi-164 cell line. CD40 expressions on the cell surface and in the cytoplasm were assessed by flow cytometry and intracellular staining assay, respectively. Also, the mRNA expression was identified by real time-PCR. The obtained results showed the high mRNA and cytoplasmic protein expression of CD40 but no surface expression. These results suggest that the Wehi-164 cell line down regulates expression of CD40 on the surface for evasion of immune system.

Entities:  

Year:  2010        PMID： 20496113      PMCID： PMC2932909          DOI： 10.1007/s10616-010-9277-x

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  17 in total

Review 1.  The CD40/CD154 receptor/ligand dyad.

Authors:  U Schönbeck; P Libby
Journal:  Cell Mol Life Sci       Date:  2001-01       Impact factor: 9.261

Review 2.  The CD40/CD40L costimulatory pathway in inflammatory bowel disease.

Authors:  S Danese; M Sans; C Fiocchi
Journal:  Gut       Date:  2004-07       Impact factor: 23.059

3.  A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory.

Authors:  Christine Bourgeois; Benedita Rocha; Corinne Tanchot
Journal:  Science       Date:  2002-09-20       Impact factor: 47.728

4.  A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes.

Authors:  T Espevik; J Nissen-Meyer
Journal:  J Immunol Methods       Date:  1986-12-04       Impact factor: 2.303

5.  Specificity of endogenous fatty acid release during tumor necrosis factor-induced apoptosis in WEHI 164 fibrosarcoma cells.

Authors:  O L Brekke; E Sagen; K S Bjerve
Journal:  J Lipid Res       Date:  1999-12       Impact factor: 5.922

6.  Tepoxalin enhances the activity of an antioxidant, pyrrolidine dithiocarbamate, in attenuating tumor necrosis factor alpha-induced apoptosis in WEHI 164 cells.

Authors:  D H Lee; J P Macintyre; G R Taylor; E Wang; R K Plante; S S Tam; B L Pope; C Y Lau
Journal:  J Pharmacol Exp Ther       Date:  1999-06       Impact factor: 4.030

7.  Dendritic cells genetically engineered to express Fas ligand induce donor-specific hyporesponsiveness and prolong allograft survival.

Authors:  W P Min; R Gorczynski; X Y Huang; M Kushida; P Kim; M Obataki; J Lei; R M Suri; M S Cattral
Journal:  J Immunol       Date:  2000-01-01       Impact factor: 5.422

8.  Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro.

Authors:  B J Sugarman; B B Aggarwal; P E Hass; I S Figari; M A Palladino; H M Shepard
Journal:  Science       Date:  1985-11-22       Impact factor: 47.728

9.  CD40 ligand (CD154) triggers a short-term CD4(+) T cell activation response that results in secretion of immunomodulatory cytokines and apoptosis.

Authors:  P J Blair; J L Riley; D M Harlan; R Abe; D K Tadaki; S C Hoffmann; L White; T Francomano; S J Perfetto; A D Kirk; C H June
Journal:  J Exp Med       Date:  2000-02-21       Impact factor: 14.307

10.  Ligation of CD40 on dendritic cells triggers production of high levels of interleukin-12 and enhances T cell stimulatory capacity: T-T help via APC activation.

Authors:  M Cella; D Scheidegger; K Palmer-Lehmann; P Lane; A Lanzavecchia; G Alber
Journal:  J Exp Med       Date:  1996-08-01       Impact factor: 14.307
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