| Literature DB >> 20495944 |
Jiewen Zhou1, Lei Huang, Jiazhang Lian, Jiayuan Sheng, Jin Cai, Zhinan Xu.
Abstract
A functional glucokinase (YqgR), N-acetylglucosamine-phosphate mutase (Agm1) and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) were synthesized simultaneously in an Escherichia coli cell-free system. These three enzymes were coupled to reconstruct a biosynthetic pathway which could produce UDP-N-acetylglucosamine with N-acetylglucosamine as the substrate.Entities:
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Year: 2010 PMID： 20495944 DOI： 10.1007/s10529-010-0315-8
Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461