Li Li1, Chengtao Li, Suhua Zhang, Shumin Zhao, Yan Liu, Yuan Lin. 1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. anneinchina@163.com
Abstract
OBJECTIVE: To genotype X-linked single nucleotide polymorphisms by TaqMan SNP genotyping assay. METHODS: 14 SNP markers on X chromosome were selected and genotyped by TaqMan SNP genotyping assay in Chinese Han population samples. RESULTS: According to the results of population studies, deviations from Hardy-Weinberg equilibrium could be found at rs1299087 SNP loci, no deviations from Hardy-Weinberg equilibrium could be found at the other 13 SNP loci. The 14 X-SNPs were high informative. The overall power of discrimination (CPD) in females and in males were 0.999998379 and 0.999899, respectively. The combined exclusion probability in paternity testing for trios and duos were 0.9983 and 0.9788, respectively. CONCLUSION: SNP typing by TaqMan SNP genotyping assay is suitable for low-throughput application. The forensic efficiency parameters showed that the 14 X-linked SNPs are highly informative and seem to be useful for forensic genetics except rs1299087.
OBJECTIVE: To genotype X-linked single nucleotide polymorphisms by TaqMan SNP genotyping assay. METHODS: 14 SNP markers on X chromosome were selected and genotyped by TaqMan SNP genotyping assay in Chinese Han population samples. RESULTS: According to the results of population studies, deviations from Hardy-Weinberg equilibrium could be found at rs1299087 SNP loci, no deviations from Hardy-Weinberg equilibrium could be found at the other 13 SNP loci. The 14 X-SNPs were high informative. The overall power of discrimination (CPD) in females and in males were 0.999998379 and 0.999899, respectively. The combined exclusion probability in paternity testing for trios and duos were 0.9983 and 0.9788, respectively. CONCLUSION: SNP typing by TaqMan SNP genotyping assay is suitable for low-throughput application. The forensic efficiency parameters showed that the 14 X-linked SNPs are highly informative and seem to be useful for forensic genetics except rs1299087.
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