The presence of dopamine-?-hydroxylase-like enzyme in the vertebrate retina.

An analysis of the dopamine-?-hydroxylase activity in various ocular tissues revealed low enzymatic activity in all the retinas analysed. Bovine and monkey retinas had the highest enzyme activity. The enzyme is also present in bovine optic nerve and pigment epithelium/choroid complex, and low levels are present in the vitreous. Immunohistochemical analysis of various retinas with an antiserum raised to bovine adrenal dopamine-?-hydroxylase-showed positive staining only in monkey and bovine retinas. In both instances dopamine-?-hydroxylase immunoreactivity was associated with cells in the ganglion cell layer. These cells are probably ganglion cells since some positive staining was also observed in the bovine optic nerve. Autoradiographical analysis of the uptake of [(3)H]noradrenaline showed that grains were associated with some structures in the ganglion cell layer. A single prominent layer of terminals at the junction of the inner nuclear and inner plexiform layer of the bovine retina was also apparent. This was considered to be due to [(3)H]noradrenaline accumulation by dopaminergic amacrine cells. The results suggest that a small number of noradrenergic and/or adrenergic ganglion cells may exist in certain retinas.