OBJECTIVE AND DESIGN: The aim of the study is to examine the expression and possible biological function of β1,4-galactosyltransferase-I (β1,4-GalT-I) in synovial tissue from rheumatoid arthritis (RA) patients. METHODS: Synovial tissue samples from twelve RA patients were stained for β1,4-GalT-I. Samples from seven patients with osteoarthritis (OA) and eight healthy people were obtained as controls. Real-time PCR or western blot analysis was used to observe the expression of β1,4-GalT-I and E-selectin. Cellular colocalization of β1,4-GalT-I, galactose-containing glycans and other molecules was analyzed by double immunofluorescence. RESULTS: Expression of β1,4-GalT-I and galactose-containing glycans increased in synovial tissue of RA patients compared with OA patients and healthy controls. Most galactose-containing glycans and β1,4-GalT-I were expressed in inflammatory cells. E-selectin overexpressed and was correlated with galactose-containing glycans in RA synovial tissue. CONCLUSION: These results suggested that β1,4-GalT-I may play an important role in the inflammatory processes in synovial tissue of patients with rheumatoid arthritis.
OBJECTIVE AND DESIGN: The aim of the study is to examine the expression and possible biological function of β1,4-galactosyltransferase-I (β1,4-GalT-I) in synovial tissue from rheumatoid arthritis (RA) patients. METHODS: Synovial tissue samples from twelve RApatients were stained for β1,4-GalT-I. Samples from seven patients with osteoarthritis (OA) and eight healthy people were obtained as controls. Real-time PCR or western blot analysis was used to observe the expression of β1,4-GalT-I and E-selectin. Cellular colocalization of β1,4-GalT-I, galactose-containing glycans and other molecules was analyzed by double immunofluorescence. RESULTS: Expression of β1,4-GalT-I and galactose-containing glycans increased in synovial tissue of RApatients compared with OA patients and healthy controls. Most galactose-containing glycans and β1,4-GalT-I were expressed in inflammatory cells. E-selectin overexpressed and was correlated with galactose-containing glycans in RA synovial tissue. CONCLUSION: These results suggested that β1,4-GalT-I may play an important role in the inflammatory processes in synovial tissue of patients with rheumatoid arthritis.
Authors: P A Klimiuk; S Sierakowski; R Latosiewicz; J P Cylwik; B Cylwik; J Skowronski; J Chwiecko Journal: Ann Rheum Dis Date: 2002-09 Impact factor: 19.103
Authors: Leo A B Joosten; Timothy R D Radstake; Erik Lubberts; Liduine A M van den Bersselaar; Piet L C M van Riel; Peter L E M van Lent; Pilar Barrera; Wim B van den Berg Journal: Arthritis Rheum Date: 2003-02