Literature DB >> 2049083

cDNA cloning of the rat O6-methylguanine-DNA-methyltransferase.

I Rahden-Staron1, F Laval.   

Abstract

A cDNA expression library was constructed from a rat hepatoma cell line ( H4 cells ) and introduced into an Escherichia coli strain ( BK2110 ) deficient in the repair of O6-methylguanine residues. Following three exposures to N-methyl-N'-nitro-N-nitrosoguanidine, a resistant colony harboring a plasmid named RMGMT was isolated. Extracts of BK2210 cells hosting the RMGMT plasmid expressed a O6-methylguanine-DNA-methyltransferase (transferase) activity and this protein had the same molecular weight as the transferase from H4 cells. The cDNA sequence of 763 bp contains an open reading frame of 630 bp encoding a protein of 209 amino acids with a calculated molecular weight of 22.2 kd. The rat protein shows 68% homology with the human transferase.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 2049083     DOI： 10.1016/0006-291x(91)91830-6

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

Review 1.  Self-destruction and tolerance in resistance of mammalian cells to alkylation damage.

Authors:  P Karran; M Bignami
Journal:  Nucleic Acids Res       Date:  1992-06-25       Impact factor: 16.971

2.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-12-11       Impact factor: 16.971

3.  Isolation and partial characterisation of a Chinese hamster O6-alkylguanine-DNA alkyltransferase cDNA.

Authors:  J A Rafferty; R H Elder; A J Watson; L Cawkwell; P M Potter; G P Margison
Journal:  Nucleic Acids Res       Date:  1992-04-25       Impact factor: 16.971

4.  Phosphorylation of methylated-DNA-protein-cysteine S-methyltransferase at serine-204 significantly increases its resistance to proteolytic digestion.

Authors:  I K Lim; T J Park; W K Paik
Journal:  Biochem J       Date:  2000-12-15       Impact factor: 3.857

5.  Requirement of the Pro-Cys-His-Arg sequence for O6-methylguanine-DNA methyltransferase activity revealed by saturation mutagenesis with negative and positive screening.

Authors:  K Ihara; H Kawate; L L Chueh; H Hayakawa; M Sekiguchi
Journal:  Mol Gen Genet       Date:  1994-05-25

6.  Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA.

Authors:  L K Liem; A Lim; B F Li
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

7.  Cloning and expresion of cDNA for rat O6-methylguanine-DNA methyltransferase.

Authors:  K Sakumi; A Shiraishi; H Hayakawa; M Sekiguchi
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

Review 8.  DNA-repair methyltransferase as a molecular device for preventing mutation and cancer.

Authors:  M Sekiguchi; Y Nakabeppu; K Sakumi; T Tuzuki
Journal:  J Cancer Res Clin Oncol       Date:  1996       Impact factor: 4.553

9.  C-terminally truncated human O6-alkylguanine-DNA alkyltransferase retains activity.

Authors:  R H Elder; J Tumelty; K T Douglas; G P Margison; J A Rafferty
Journal:  Biochem J       Date:  1992-08-01       Impact factor: 3.857

10.  Construction and characterization of mutants of Salmonella typhimurium deficient in DNA repair of O6-methylguanine.

Authors:  M Yamada; B Sedgwick; T Sofuni; T Nohmi
Journal:  J Bacteriol       Date:  1995-03       Impact factor: 3.490
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.