Literature DB >> 20490755

Effective solubilization and single-step purification of Bacillus licheniformis alpha-amylase from insoluble aggregates.

N Rashid1, N Ahmed, M Saleem Haider, I Haque.   

Abstract

A high level expression of thermostable alpha-amylase gene from Bacillus licheniformis in Escherichia coli was obtained. The recombinant enzyme was mainly produced in the form of insoluble aggregates. The enzyme was solubilized without using denaturing agents and purified to homogeneity in a single step by ion exchange chromatography. The enzyme was purified 138-fold with a final yield of 349 %; the specific activity of the purified enzyme was 1343 U/mg.

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Year:  2010        PMID： 20490755     DOI： 10.1007/s12223-010-0020-y

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  26 in total

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Journal:  Nat Biotechnol       Date:  1996-10       Impact factor: 54.908

2.  Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli.

Authors:  J G Thomas; F Baneyx
Journal:  Protein Expr Purif       Date:  1997-12       Impact factor: 1.650

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Authors:  L L Lin; W H Hsu
Journal:  Lett Appl Microbiol       Date:  1997-05       Impact factor: 2.858

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Authors:  M Gribskov; R R Burgess
Journal:  Gene       Date:  1983-12       Impact factor: 3.688

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Authors:  Y S Cheng
Journal:  Biochem Biophys Res Commun       Date:  1983-02-28       Impact factor: 3.575

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Authors:  J L Marco; L A Bataus; F F Valência; C J Ulhoa; S Astolfi-Filho; C R Felix
Journal:  Appl Microbiol Biotechnol       Date:  1996-02       Impact factor: 4.813

7.  Effects of signal peptide mutations on processing of Bacillus stearothermophilus alpha-amylase in Escherichia coli.

Authors:  I Suominen; P Meyer; C Tilgmann; T Glumoff; V Glumoff; J Käpylä; P Mäntsälä
Journal:  Microbiology       Date:  1995-03       Impact factor: 2.777

8.  Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus.

Authors:  Linley R Schofield; Mark L Patchett; Emily J Parker
Journal:  Protein Expr Purif       Date:  2004-03       Impact factor: 1.650

Review 9.  Recent developments in heterologous protein production in Escherichia coli.

Authors:  R C Hockney
Journal:  Trends Biotechnol       Date:  1994-11       Impact factor: 19.536

10.  High-level production of the EcoRI endonuclease under the control of the pL promoter of bacteriophage lambda.

Authors:  J Botterman; M Zabeau
Journal:  Gene       Date:  1985       Impact factor: 3.688
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  1 in total

1.  Property Improvement of α-Amylase from
Bacillus stearothermophilus by Deletion of Amino Acid
Residues Arginine 179 and Glycine 180.

Authors:  Yuanming Gai; Jingqi Chen; Shibin Zhang; Beiwei Zhu; Dawei Zhang
Journal:  Food Technol Biotechnol       Date:  2018-03       Impact factor: 3.918
  1 in total

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