| Literature DB >> 20486120 |
Lifeng Peng1, Eugene A Kapp, David Fenyö, Min-Seok Kwon, Pu Jiang, Songfeng Wu, Ying Jiang, Marie-Isabel Aguilar, Nikhat Ahmed, Mark S Baker, Zongwei Cai, Yu-Ju Chen, Phan Van Chi, Maxey C M Chung, Fuchu He, Alice C L Len, Pao-Chi Liao, Kazuyuki Nakamura, Sai Ming Ngai, Young-Ki Paik, Tai-Long Pan, Terence C W Poon, Ghasem Hosseini Salekdeh, Richard J Simpson, Ravi Sirdeshmukh, Chantragan Srisomsap, Jisnuson Svasti, Yu-Chang Tyan, Florian S Dreyer, Danyl McLauchlan, Pisana Rawson, T William Jordan.
Abstract
The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.Entities:
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Year: 2010 PMID： 20486120 PMCID： PMC3891941 DOI： 10.1002/pmic.201000126
Source DB: PubMed Journal: Proteomics ISSN： 1615-9853 Impact factor: 3.984