INTRODUCTION: Pulp stones are frequently formed as a pathologic calcification product in dental pulp tissues, but the pathogenesis is poorly understood. We previously found that osteopontin (OPN) was produced by dental pulp cells, and its expression was associated with formation of the pulp stone matrix. It was reported that amorphous calcification appeared in the dental pulp of diabetic patients. The aim of this study was to determine the relationship between OPN expression and pathologic calcification in rat diabetic pulp. METHODS: The effect of glucose on OPN production and alkaline phosphatase activity in cultured rat dental pulp cells (RPC-C2A) was investigated, and then dental pulp calcification and OPN expression in diabetic rats were determined and compared with those in healthy rats by histologic and immunohistochemical analyses. RESULTS: In RPC-C2A cells, biochemical analysis showed that a high concentration of glucose (50 mmol/L) increased OPN protein production and alkaline phosphatase activity 1.3-fold and 1.5-fold, respectively. Histologic observations showed more calcified particles in dental pulp tissues in diabetic than in nondiabetic rats. Moreover, a thickened layer of predentin was formed in the radicular pulp of diabetic rats. OPN was more strongly stained around the calcified particles and in the odontoblast zone under the thickened predentin in diabetic rats. CONCLUSIONS: OPN might be a key molecule involved in the increase of pathologic pulp calcifications, which are frequently observed in diabetic patients. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
INTRODUCTION: Pulp stones are frequently formed as a pathologic calcification product in dental pulp tissues, but the pathogenesis is poorly understood. We previously found that osteopontin (OPN) was produced by dental pulp cells, and its expression was associated with formation of the pulp stone matrix. It was reported that amorphous calcification appeared in the dental pulp of diabeticpatients. The aim of this study was to determine the relationship between OPN expression and pathologic calcification in ratdiabetic pulp. METHODS: The effect of glucose on OPN production and alkaline phosphatase activity in cultured rat dental pulp cells (RPC-C2A) was investigated, and then dental pulp calcification and OPN expression in diabeticrats were determined and compared with those in healthy rats by histologic and immunohistochemical analyses. RESULTS: In RPC-C2A cells, biochemical analysis showed that a high concentration of glucose (50 mmol/L) increased OPN protein production and alkaline phosphatase activity 1.3-fold and 1.5-fold, respectively. Histologic observations showed more calcified particles in dental pulp tissues in diabetic than in nondiabetic rats. Moreover, a thickened layer of predentin was formed in the radicular pulp of diabeticrats. OPN was more strongly stained around the calcified particles and in the odontoblast zone under the thickened predentin in diabeticrats. CONCLUSIONS:OPN might be a key molecule involved in the increase of pathologic pulp calcifications, which are frequently observed in diabeticpatients. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Authors: Yandy Gonzalez Marrero; Yoshifumi Kobayashi; Mohammad Saqib Ihsan; Lisa A Pilch; Liyaa Chen; Shuying Jiang; Yi Ye; Daniel H Fine; Carla Y Falcon; Paul A Falcon; Craig S Hirschberg; Emi Shimizu Journal: J Endod Date: 2021-11-13 Impact factor: 4.171
Authors: Luciana B Ferreira; Raquel T Lima; Ana Clara Santos da Fonseca Bastos; Andreia M Silva; Catarina Tavares; Ana Pestana; Elisabete Rios; Catarina Eloy; Manuel Sobrinho-Simões; Etel R P Gimba; Paula Soares Journal: Int J Mol Sci Date: 2018-09-30 Impact factor: 5.923