Literature DB >> 2047367

Recognition of recombinant Toxoplasma gondii antigens by human sera in an ELISA.

A M Tenter1, A M Johnson.   

Abstract

We developed an enzyme-linked immunosorbent assay (ELISA) that uses one of two recombinant polypeptides, termed H4/GST and H11/GST, as diagnostic antigens for the detection of antibodies to Toxoplasma gondii in human sera. A total of 59 sera from humans with acute toxoplasmosis, 194 sera from patients with chronic toxoplasmosis, and 151 sera from subjects who were not infected with T. gondii were examined. In all, 68% of the sera from humans with acute toxoplasmosis reacted positively with one or both recombinant T. gondii antigens. By contrast, only 14% of those from patients with chronic toxoplasmosis recognized H4/GST or H11/GST. None of the sera from humans who were not infected with T. gondii, including patients with echinococcosis, entamoebosis, toxocarosis, trichinellosis, glandular fever, or rheumatoid arthritis, recognized H4/GST or H11/GST.

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Year:  1991        PMID： 2047367     DOI： 10.1007/bf00930858

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  21 in total

1.  Detection of nucleoside triphosphate hydrolase as a circulating antigen in sera of mice infected with Toxoplasma gondii.

Authors:  T Asai; T J Kim; M Kobayashi; S Kojima
Journal:  Infect Immun       Date:  1987-05       Impact factor: 3.441

2.  Western blot analysis of the antibody response of patients with AIDS and toxoplasma encephalitis: antigenic diversity among Toxoplasma strains.

Authors:  L M Weiss; S A Udem; H Tanowitz; M Wittner
Journal:  J Infect Dis       Date:  1988-01       Impact factor: 5.226

3.  Cloning, expression and nucleotide sequence of the gene fragment encoding an antigenic portion of the nucleoside triphosphate hydrolase of Toxoplasma gondii.

Authors:  A M Johnson; S Illana; P J McDonald; T Asai
Journal:  Gene       Date:  1989-12-21       Impact factor: 3.688

4.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Authors:  D B Smith; K S Johnson
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

5.  Human antibody response to the nucleoside triphosphate hydrolase of Toxoplasma gondii.

Authors:  A M Tenter; A M Johnson
Journal:  J Immunoassay       Date:  1990

6.  Purification and characterization of a cloned protease-resistant Treponema pallidum-specific antigen.

Authors:  T E Fehniger; A M Walfield; T M Cunningham; J D Radolf; J N Miller; M A Lovett
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

7.  Defined Plasmodium falciparum antigens in malaria serology.

Authors:  M S Gabra; D Grossiord; L H Perrin; A Shaw; A Cheung; I A McGregor
Journal:  Bull World Health Organ       Date:  1986       Impact factor: 9.408

8.  Immunoblot analysis of Toxoplasma gondii antigens by human immunoglobulins G, M, and A antibodies at different stages of infection.

Authors:  P Partanen; H J Turunen; R T Paasivuo; P O Leinikki
Journal:  J Clin Microbiol       Date:  1984-07       Impact factor: 5.948

9.  Antigen(s) responsible for immunoglobulin G responses specific for the acute stage of Toxoplasma infection in humans.

Authors:  Y Suzuki; P Thulliez; G Desmonts; J S Remington
Journal:  J Clin Microbiol       Date:  1988-05       Impact factor: 5.948

10.  Recombinant polypeptides for serology of malaria.

Authors:  I K Srivastava; B Takacs; P Caspers; U Certa; I A McGregor; J Scaife; L H Perrin
Journal:  Trans R Soc Trop Med Hyg       Date:  1989 May-Jun       Impact factor: 2.184
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  20 in total

1.  Enzyme-linked immunosorbent assays using the recombinant dense granule antigens GRA6 and GRA1 of Toxoplasma gondii for detection of immunoglobulin G antibodies.

Authors:  L Lecordier; M P Fourmaux; C Mercier; E Dehecq; E Masy; M F Cesbron-Delauw
Journal:  Clin Diagn Lab Immunol       Date:  2000-07

2.  Fractionation of membrane components from tachyzoite forms of Toxoplasma gondii: differential recognition by immunoglobulin M (IgM) and IgG present in sera from patients with acute or chronic toxoplasmosis.

Authors:  M Giraldo; H Cannizzaro; M A Ferguson; I C Almeida; R T Gazzinelli
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

3.  Serodiagnosis of recently acquired Toxoplasma gondii infection with a recombinant antigen.

Authors:  S Li; G Maine; Y Suzuki; F G Araujo; G Galvan; J S Remington; S Parmley
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

4.  Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii.

Authors:  S F Parmley; G D Sgarlato; J Mark; J B Prince; J S Remington
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

5.  Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzyme-linked immunosorbent assay with a combination of recombinant antigens.

Authors:  S Li; G Galvan; F G Araujo; Y Suzuki; J S Remington; S Parmley
Journal:  Clin Diagn Lab Immunol       Date:  2000-09

6.  Immunoglobulin M (IgM)-glycoinositolphospholipid enzyme-linked immunosorbent assay: an immunoenzymatic assay for discrimination between patients with acute toxoplasmosis and those with persistent parasite-specific IgM antibodies.

Authors:  Mónica Giraldo; Ricardo W D Portela; Mirian Snege; Paulo G Leser; Mário E Camargo; José Roberto Mineo; Ricardo T Gazzinelli
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

7.  Limited value of assays using detection of immunoglobulin G antibodies to the two recombinant dense granule antigens, GRA1 and GRA6 Nt of Toxoplasma gondii, for distinguishing between acute and chronic infections in pregnant women.

Authors:  Josette Ferrandiz; Corinne Mercier; Martine Wallon; Stéphane Picot; Marie-France Cesbron-Delauw; François Peyron
Journal:  Clin Diagn Lab Immunol       Date:  2004-11

8.  Use of a recombinant Dictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis.

Authors:  T Schnieder
Journal:  Parasitol Res       Date:  1992       Impact factor: 2.289

9.  Evaluation of recombinant dense granule antigen 7 (GRA7) of Toxoplasma gondii for detection of immunoglobulin G antibodies and analysis of a major antigenic domain.

Authors:  D Jacobs; M Vercammen; E Saman
Journal:  Clin Diagn Lab Immunol       Date:  1999-01

10.  Serodiagnosis of toxoplasmosis by using a recombinant form of the 54-kilodalton rhoptry antigen expressed in Escherichia coli.

Authors:  P van Gelder; F Bosman; F de Meuter; H van Heuverswyn; P Hérion
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948
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