OBJECTIVE AND DESIGN: A 6-month, randomized clinical study was conducted to evaluate the effect of a ribonuclease-enriched lactoferrin (R-ELF) supplement on the circulating cytokine levels and bone health of postmenopausal women. SUBJECTS:Thirty-eight healthy postmenopausal women, aged 45-60 years, were randomized into placebo and R-ELF groups. TREATMENT: The R-ELF group was supplemented with R-ELF (2 × 125 mg/day) and calcium (100% RDA), while the placebo group received only the calcium supplement. METHODS:Serum levels of receptor activator for NF-κB ligand (RANKL), C-reactive protein (CRP) and various pro- and anti-inflammatory cytokines were determined by ELISA. RESULTS:Pro-inflammatory cytokines IL-6 and TNF-α decreased significantly (-44 and -10%, respectively) while anti-inflammatory IL-10 increased (140%) due to R-ELF supplementation at the end of study. RANKL and CRP were modestly reduced (-50%) relative to their placebo levels, although RANKL elevated initially. CONCLUSIONS:R-ELF supplementation showed beneficial effects towards improvement of inflammatory status in postmenopausal women.
RCT Entities:
OBJECTIVE AND DESIGN: A 6-month, randomized clinical study was conducted to evaluate the effect of a ribonuclease-enriched lactoferrin (R-ELF) supplement on the circulating cytokine levels and bone health of postmenopausal women. SUBJECTS: Thirty-eight healthy postmenopausal women, aged 45-60 years, were randomized into placebo and R-ELF groups. TREATMENT: The R-ELF group was supplemented with R-ELF (2 × 125 mg/day) and calcium (100% RDA), while the placebo group received only the calcium supplement. METHODS: Serum levels of receptor activator for NF-κB ligand (RANKL), C-reactive protein (CRP) and various pro- and anti-inflammatory cytokines were determined by ELISA. RESULTS: Pro-inflammatory cytokines IL-6 and TNF-α decreased significantly (-44 and -10%, respectively) while anti-inflammatory IL-10 increased (140%) due to R-ELF supplementation at the end of study. RANKL and CRP were modestly reduced (-50%) relative to their placebo levels, although RANKL elevated initially. CONCLUSIONS: R-ELF supplementation showed beneficial effects towards improvement of inflammatory status in postmenopausal women.
Authors: Marloes van Splunter; Olaf Perdijk; Henriëtte Fick-Brinkhof; Anouk L Feitsma; Esther G Floris-Vollenbroek; Ben Meijer; Sylvia Brugman; Huub F J Savelkoul; Els van Hoffen; R J Joost van Neerven Journal: Front Immunol Date: 2018-11-20 Impact factor: 7.561
Authors: Tamara N Dunn; Alison H Keenan; Anthony P Thomas; John W Newman; Sean H Adams Journal: Nutr Metab (Lond) Date: 2014-05-30 Impact factor: 4.169
Authors: Andrea Ticinesi; Tiziana Meschi; Fulvio Lauretani; Giovanna Felis; Fabrizio Franchi; Carlo Pedrolli; Michela Barichella; Giuseppe Benati; Sergio Di Nuzzo; Gian Paolo Ceda; Marcello Maggio Journal: Nutrients Date: 2016-03-29 Impact factor: 5.717