OBJECTIVES: The present study was conducted to monitor the expression of pancreas and duodenal homeobox gene (PDX-1) for assessing beta-cell function in islets from patients with chronic pancreatitis (CP). METHODS: Islets isolated from the pancreata of 40 surgical patients categorized as control group, patients with mild CP, and patients with advanced CP were assessed for their yield, size, and glucose-stimulated insulin secretion. Expressions of genes coding for PDX-1, insulin, and glucagon were simultaneously monitored by reverse transcription polymerase chain reaction and confirmed by immunohistochemistry. RESULTS: In comparison with the control group (2673 +/- 592 islet equivalents [IEq]/g), islet yield did not differ much in the patients with mild CP (2344 +/- 738 IEq/g) but was significantly reduced (P < 0.0001) in the patients with advanced CP (731 +/- 167 IEq/g). Although the marginal decrease in islet size observed in the patients with mild CP was not significantly different from that observed in the control group, there was a 58% decrease observed in the patients with advanced CP that was also accompanied by a significant reduction in beta-cell mass (P < 0.05). The expression of insulin and PDX-1 genes, but not of glucagon, was significantly reduced in the patients with advanced CP as confirmed by immunohistochemistry. Islets obtained from the patients with advanced CP retained 53% glucose-stimulated insulin secretion function in comparison with those of the control group. CONCLUSION: The results indicate that beta-cell dysfunction during progression of CP correlates with the decrease in PDX-1 gene expression.
OBJECTIVES: The present study was conducted to monitor the expression of pancreas and duodenal homeobox gene (PDX-1) for assessing beta-cell function in islets from patients with chronic pancreatitis (CP). METHODS: Islets isolated from the pancreata of 40 surgical patients categorized as control group, patients with mild CP, and patients with advanced CP were assessed for their yield, size, and glucose-stimulated insulin secretion. Expressions of genes coding for PDX-1, insulin, and glucagon were simultaneously monitored by reverse transcription polymerase chain reaction and confirmed by immunohistochemistry. RESULTS: In comparison with the control group (2673 +/- 592 islet equivalents [IEq]/g), islet yield did not differ much in the patients with mild CP (2344 +/- 738 IEq/g) but was significantly reduced (P < 0.0001) in the patients with advanced CP (731 +/- 167 IEq/g). Although the marginal decrease in islet size observed in the patients with mild CP was not significantly different from that observed in the control group, there was a 58% decrease observed in the patients with advanced CP that was also accompanied by a significant reduction in beta-cell mass (P < 0.05). The expression of insulin and PDX-1 genes, but not of glucagon, was significantly reduced in the patients with advanced CP as confirmed by immunohistochemistry. Islets obtained from the patients with advanced CP retained 53% glucose-stimulated insulin secretion function in comparison with those of the control group. CONCLUSION: The results indicate that beta-cell dysfunction during progression of CP correlates with the decrease in PDX-1 gene expression.
Authors: M Sasikala; R Talukdar; P Pavan kumar; G Radhika; G V Rao; R Pradeep; C Subramanyam; D Nageshwar Reddy Journal: Dig Dis Sci Date: 2012-03-02 Impact factor: 3.199
Authors: G V Rao; R Pradeep; M Sasikala; P Pavan Kumar; V V Krishna; G Mahesh Shetty; R Talukdar; M Tandan; R Jagadeesh; D Nageshwar Reddy Journal: Indian J Gastroenterol Date: 2018-10-01
Authors: Mark O Goodarzi; Tanvi Nagpal; Phil Greer; Jinrui Cui; Yii-Der I Chen; Xiuqing Guo; James S Pankow; Jerome I Rotter; Samer Alkaade; Stephen T Amann; John Baillie; Peter A Banks; Randall E Brand; Darwin L Conwell; Gregory A Cote; Christopher E Forsmark; Timothy B Gardner; Andres Gelrud; Nalini Guda; Jessica LaRusch; Michele D Lewis; Mary E Money; Thiruvengadam Muniraj; Georgios I Papachristou; Joseph Romagnuolo; Bimaljit S Sandhu; Stuart Sherman; Vikesh K Singh; C Mel Wilcox; Stephen J Pandol; Walter G Park; Dana K Andersen; Melena D Bellin; Phil A Hart; Dhiraj Yadav; David C Whitcomb Journal: Clin Transl Gastroenterol Date: 2019-07 Impact factor: 4.488