Literature DB >> 20463168

Comparison of single-copy and multicopy real-time PCR targets for detection of Mycobacterium tuberculosis in paraffin-embedded tissue.

Robert F Luo1, Michael D Scahill, Niaz Banaei.   

Abstract

Real-time PCR can rapidly identify Mycobacterium tuberculosis in paraffin-embedded tissue in the absence of microbiological culture. In a comparison of single-copy and multicopy PCR targets in 70 tissue samples, the sensitivities were 26% and 54%, respectively, with 100% specificity. Sensitivity was 75% for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.

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Year:  2010        PMID： 20463168      PMCID： PMC2897508          DOI： 10.1128/JCM.02449-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Real-time PCR assay for improved detection of Mycobacterium tuberculosis complex in paraffin-embedded tissues.

Authors:  D Hillemann; J Galle; E Vollmer; E Richter
Journal:  Int J Tuberc Lung Dis       Date:  2006-03       Impact factor: 2.373

2.  Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues.

Authors:  Christiane Schewe; Torsten Goldmann; Marianne Grosser; Albert Zink; Karsten Schlüns; Stefan Pahl; Timo Ulrichs; Stefan H E Kaufmann; Andreas Nerlich; Gustavo B Baretton; Manfred Dietel; Ekkehard Vollmer; Iver Petersen
Journal:  Virchows Arch       Date:  2005-06-21       Impact factor: 4.064

3.  Development of a real-time PCR-based method for rapid differential identification of Mycobacterium species.

Authors:  S Y Lim; B-J Kim; M-K Lee; K Kim
Journal:  Lett Appl Microbiol       Date:  2007-11-19       Impact factor: 2.858

4.  Rapid Identification of Mycobacterium tuberculosis and nontuberculous mycobacteria by multiplex, real-time PCR.

Authors:  E T Richardson; D Samson; N Banaei
Journal:  J Clin Microbiol       Date:  2009-03-18       Impact factor: 5.948

5.  Differentiation of sarcoidosis from tuberculosis using real-time PCR assay for the detection and quantification of Mycobacterium tuberculosis.

Authors:  Y Zhou; H P Li; Q H Li; H Zheng; R X Zhang; G Chen; R P Baughman
Journal:  Sarcoidosis Vasc Diffuse Lung Dis       Date:  2008-12       Impact factor: 0.670

6.  Effects of tissue handling and processing steps on PCR for detection of Mycobacterium tuberculosis in formalin-fixed paraffin-embedded samples.

Authors:  Denise Barcelos; Marcello F Franco; Sylvia Cardoso Leão
Journal:  Rev Inst Med Trop Sao Paulo       Date:  2008 Nov-Dec       Impact factor: 1.846

7.  Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology.

Authors:  Danica Helb; Martin Jones; Elizabeth Story; Catharina Boehme; Ellen Wallace; Ken Ho; JoAnn Kop; Michelle R Owens; Richard Rodgers; Padmapriya Banada; Hassan Safi; Robert Blakemore; N T Ngoc Lan; Edward C Jones-López; Michael Levi; Michele Burday; Irene Ayakaka; Roy D Mugerwa; Bill McMillan; Emily Winn-Deen; Lee Christel; Peter Dailey; Mark D Perkins; David H Persing; David Alland
Journal:  J Clin Microbiol       Date:  2009-10-28       Impact factor: 5.948

8.  Use of the real-time PCR assay in conjunction with MagNA Pure for the detection of mycobacterial DNA from fixed specimens.

Authors:  Safedin H Beqaj; Randy Flesher; Gina R Walker; Samuel A Smith
Journal:  Diagn Mol Pathol       Date:  2007-09

9.  Multiplex real-time PCR assay for rapid identification of Mycobacterium tuberculosis complex members to the species level.

Authors:  Benjamin A Pinsky; Niaz Banaei
Journal:  J Clin Microbiol       Date:  2008-05-28       Impact factor: 5.948

10.  Molecular differentiation of Mycobacterium tuberculosis strains without IS6110 insertions.

Authors:  Kerry H Lok; William H Benjamin; Michael E Kimerling; Virginia Pruitt; Monica Lathan; Jafar Razeq; Nancy Hooper; Wendy Cronin; Nancy E Dunlap
Journal:  Emerg Infect Dis       Date:  2002-11       Impact factor: 6.883
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  9 in total

1.  Simple Real-Time PCR and Amplicon Sequencing Method for Identification of Plasmodium Species in Human Whole Blood.

Authors:  Martina I Lefterova; Indre Budvytiene; Johanna Sandlund; Anna Färnert; Niaz Banaei
Journal:  J Clin Microbiol       Date:  2015-05-13       Impact factor: 5.948

2.  Simple Processing of Formalin-Fixed Paraffin-Embedded Tissue for Accurate Testing with the Xpert MTB/RIF Assay.

Authors:  Indre Budvytiene; Niaz Banaei
Journal:  J Clin Microbiol       Date:  2020-02-24       Impact factor: 5.948

Review 3.  Novel developments in the epidemic of human immunodeficiency virus and tuberculosis coinfection.

Authors:  Asha Anandaiah; Keertan Dheda; Joseph Keane; Henry Koziel; David A J Moore; Naimish R Patel
Journal:  Am J Respir Crit Care Med       Date:  2010-12-22       Impact factor: 21.405

4.  Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens.

Authors:  Hongwei Shen; Bingqing Zhu; Shulian Wang; Haolian Mo; Ji Wang; Jin Li; Chen Zhang; Huashu Zeng; Li Guan; Weixian Shi; Yong Zhang; Xuejun Ma
Journal:  Front Microbiol       Date:  2015-05-28       Impact factor: 5.640

5.  Delayed Diagnosis of Tuberculous Meningitis Misdiagnosed as Herpes Simplex Virus-1 Encephalitis With the FilmArray Syndromic Polymerase Chain Reaction Panel.

Authors:  Carlos A Gomez; Benjamin A Pinsky; Anne Liu; Niaz Banaei
Journal:  Open Forum Infect Dis       Date:  2016-12-07       Impact factor: 3.835

6.  Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens.

Authors:  Pratt Kolia-Diafouka; Sylvain Godreuil; Arnaud Bourdin; Severine Carrère-Kremer; Laurent Kremer; Philippe Van de Perre; Edouard Tuaillon
Journal:  Front Microbiol       Date:  2018-09-25       Impact factor: 5.640

7.  DNA-based quantification and counting of transmission stages provides different but complementary parasite load estimates: an example from rodent coccidia (Eimeria).

Authors:  Víctor Hugo Jarquín-Díaz; Alice Balard; Susana Carolina Martins Ferreira; Vivian Mittné; Julia Mari Murata; Emanuel Heitlinger
Journal:  Parasit Vectors       Date:  2022-02-04       Impact factor: 3.876

Review 8.  Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis.

Authors:  Emmanuel O Babafemi; Benny P Cherian; Lee Banting; Graham A Mills; Kandala Ngianga
Journal:  Syst Rev       Date:  2017-10-25

9.  Targeting Multicopy Prophage Genes for the Increased Detection of Borrelia burgdorferi Sensu Lato (s.l.), the Causative Agents of Lyme Disease, in Blood.

Authors:  Jinyu Shan; Ying Jia; Louis Teulières; Faizal Patel; Martha R J Clokie
Journal:  Front Microbiol       Date:  2021-03-15       Impact factor: 5.640
  9 in total

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