| Literature DB >> 20457160 |
Erica Houthuys1, Kiavash Movahedi, Patrick De Baetselier, Jo A Van Ginderachter, Peter Brouckaert.
Abstract
Studies in mice have advanced our understanding of the contribution of monocytes to inflammation and immunity. However, the lack of a straightforward and reliable method for the isolation of mouse blood monocytes remains a hurdle. Here we describe a fast and easy method for isolating monocytes from mouse blood based on immuno-magnetic labeling. By negative selection we were able to isolate enriched fractions (>50% purity) of unlabeled monocytes. A subsequent positive selection step resulted in purities of > or =90% viable and functional monocytes. This method is of general use for studying mouse monocyte biology. 2010 Elsevier B.V. All rights reserved.Entities:
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Year: 2010 PMID： 20457160 DOI： 10.1016/j.jim.2010.04.004
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303