| Literature DB >> 20457127 |
Yi-Qun Geng1, Ji-Tian Guan, Xiao-Hu Xu, Yu-Cai Fu.
Abstract
To investigate the activity of senescence-associated beta-galactosidase (SA-beta-GAL) in the hippocampus of aging rats. Hippocampi of 6-, 18-, and 24-month-old rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL in cultured hippocampal neurons. The activity of SA-beta-GAL doubled in hippocampal pyramidal cells of the CA3 region in rats between 6 and 18 months (14.57+/-2.74% vs. 31.66+/-14.12% SA-beta-GAL-positive, respectively), and reached 50.76+/-14.41% positive at 24 months. The activity of SA-beta-GAL also increased as a function of time upon prolonged culture of cultured hippocampal neurons with 95% of cells being SA-beta-GAL-positive at 20 days in vitro. Interestingly, no SA-beta-GAL-positive cells were found in neurons of the hippocampal dentate gyrus, a neurogenic region of the brain, at any age examined. SA-beta-GAL can be used as a senescence biomarker in determining senescent neurons in hippocampal pyramidal cells of the CA3 region in advanced aging. (c) 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20457127 DOI： 10.1016/j.bbrc.2010.05.011
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575