Literature DB >> 20453121

NtcA from Microcystis aeruginosa PCC 7806 is autoregulatory and binds to the microcystin promoter.

H P Ginn1, L A Pearson, B A Neilan.   

Abstract

NtcA is a transcription factor that has been found in a diverse range of cyanobacteria. This nitrogen-controlled factor was focused on as a key component in the yet-to-be-deciphered regulatory network controlling microcystin production. Adaptor-mediated PCR was utilized to isolate the ntcA gene from Microcystis aeruginosa PCC 7806. This gene was cloned, and the recombinant (His-tagged) protein was overexpressed and purified for use in mobility shift assays to analyze NtcA binding to putative sites identified in the microcystin mcyA/D promoter region. Autoregulation of NtcA in M. aeruginosa was shown via NtcA binding in the upstream ntcA promoter region. The observation of binding of NtcA to the mcyA/D promoter region has direct relevance for the regulation of microcystin biosynthesis, as transcription of the mcyABCDEFGHIJ gene cluster appears to be under direct control of nitrogen.

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Year:  2010        PMID： 20453121      PMCID： PMC2897459          DOI： 10.1128/AEM.01862-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  32 in total

1.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

Authors:  K J Livak; T D Schmittgen
Journal:  Methods       Date:  2001-12       Impact factor: 3.608

2.  NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators.

Authors:  M A Vega-Palas; E Flores; A Herrero
Journal:  Mol Microbiol       Date:  1992-07       Impact factor: 3.501

3.  Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection.

Authors:  T F Wei; T S Ramasubramanian; F Pu; J W Golden
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

4.  Cloning, sequencing, and regulation of the glutathione reductase gene from the cyanobacterium Anabaena PCC 7120.

Authors:  F Jiang; U Hellman; G E Sroga; B Bergman; B Mannervik
Journal:  J Biol Chem       Date:  1995-09-29       Impact factor: 5.157

5.  General distribution of the nitrogen control gene ntcA in cyanobacteria.

Authors:  J E Frías; A Mérida; A Herrero; J Martín-Nieto; E Flores
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

6.  Immuno-gold localization of hepatotoxins in cyanobacterial cells.

Authors:  L Shi; W W Carmichael; I Miller
Journal:  Arch Microbiol       Date:  1995-01       Impact factor: 2.552

7.  Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942.

Authors:  T Omata; X Andriesse; A Hirano
Journal:  Mol Gen Genet       Date:  1993-01

8.  Microcystin uptake and inhibition of protein phosphatases: effects of chemoprotectants and self-inhibition in relation to known hepatic transporters.

Authors:  M Runnegar; N Berndt; N Kaplowitz
Journal:  Toxicol Appl Pharmacol       Date:  1995-10       Impact factor: 4.219

9.  Iron-stimulated toxin production in Microcystis aeruginosa.

Authors:  H Utkilen; N Gjølme
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

10.  Molecular mechanism for the operation of nitrogen control in cyanobacteria.

Authors:  I Luque; E Flores; A Herrero
Journal:  EMBO J       Date:  1994-06-15       Impact factor: 11.598
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  27 in total

1.  Molecular response of the bloom-forming cyanobacterium, Microcystis aeruginosa, to phosphorus limitation.

Authors:  Matthew J Harke; Dianna L Berry; James W Ammerman; Christopher J Gobler
Journal:  Microb Ecol       Date:  2011-07-01       Impact factor: 4.552

2.  Harmful cyanobacterial blooms: causes, consequences, and controls.

Authors:  Hans W Paerl; Timothy G Otten
Journal:  Microb Ecol       Date:  2013-01-13       Impact factor: 4.552

3.  Comparative protein expression in different strains of the bloom-forming cyanobacterium Microcystis aeruginosa.

Authors:  Ralitza Alexova; Paul A Haynes; Belinda C Ferrari; Brett A Neilan
Journal:  Mol Cell Proteomics       Date:  2011-05-24       Impact factor: 5.911

4.  Effects of intermediate metabolite carboxylic acids of TCA cycle on Microcystis with overproduction of phycocyanin.

Authors:  Shijie Bai; Jingcheng Dai; Ming Xia; Jing Ruan; Hehong Wei; Dianzhen Yu; Ronghui Li; Hongmei Jing; Chunyuan Tian; Lirong Song; Dongru Qiu
Journal:  Environ Sci Pollut Res Int       Date:  2014-10-24       Impact factor: 4.223

Review 5.  A review on factors affecting microcystins production by algae in aquatic environments.

Authors:  Ruihua Dai; Pinfei Wang; Peili Jia; Yi Zhang; Xincheng Chu; Yifei Wang
Journal:  World J Microbiol Biotechnol       Date:  2016-02-13       Impact factor: 3.312

6.  Variation of Microcystis and microcystins coupling nitrogen and phosphorus nutrients in Lake Erhai, a drinking-water source in Southwest Plateau, China.

Authors:  Gongliang Yu; Yongguang Jiang; Gaofei Song; Wenhua Tan; Mengling Zhu; Renhui Li
Journal:  Environ Sci Pollut Res Int       Date:  2014-05-02       Impact factor: 4.223

7.  Hormesis effects of amoxicillin on growth and cellular biosynthesis of Microcystis aeruginosa at different nitrogen levels.

Authors:  Ying Liu; Xiao Chen; Jian Zhang; Baoyu Gao
Journal:  Microb Ecol       Date:  2014-11-12       Impact factor: 4.552

8.  Transcriptomics-aided dissection of the intracellular and extracellular roles of microcystin in Microcystis aeruginosa PCC 7806.

Authors:  A Katharina Makower; J Merijn Schuurmans; Detlef Groth; Yvonne Zilliges; Hans C P Matthijs; Elke Dittmann
Journal:  Appl Environ Microbiol       Date:  2014-11-07       Impact factor: 4.792

9.  Microcystin production and regulation under nutrient stress conditions in toxic microcystis strains.

Authors:  Juliana S M Pimentel; Alessandra Giani
Journal:  Appl Environ Microbiol       Date:  2014-07-18       Impact factor: 4.792

Review 10.  Global solutions to regional problems: Collecting global expertise to address the problem of harmful cyanobacterial blooms. A Lake Erie case study.

Authors:  George S Bullerjahn; Robert M McKay; Timothy W Davis; David B Baker; Gregory L Boyer; Lesley V D'Anglada; Gregory J Doucette; Jeff C Ho; Elena G Irwin; Catherine L Kling; Raphael M Kudela; Rainer Kurmayer; Anna M Michalak; Joseph D Ortiz; Timothy G Otten; Hans W Paerl; Boqiang Qin; Brent L Sohngen; Richard P Stumpf; Petra M Visser; Steven W Wilhelm
Journal:  Harmful Algae       Date:  2016-04       Impact factor: 4.273
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