OBJECTIVE: Preimplantation factor (PIF) is a novel, 15 amino acid peptide, secreted by viable embryos. This study aims to elucidate PIF's effects in human endometrial stromal cells (HESC) decidualized by estrogen and progestin, which mimics the preimplantation milieu, and in first-trimester decidua cultures (FTDC). STUDY DESIGN: HESC or FTDC were incubated with 100 nmol/L synthetic PIF or vehicle control. Global gene expression was analyzed using microarray and pathway analysis. Proteins were analyzed using quantitative mass spectrometry, and PIF binding by protein array. RESULTS: Gene and proteomic analysis demonstrate that PIF affects immune, adhesion, and apoptotic pathways. Significant up-regulation in HESC (fold change) include: nuclear factor-k-beta activation via interleukin-1 receptor-associated kinase binding protein 1 (53); Toll-like receptor 5 (9); FK506 binding protein 15, 133kDa protein (2.3); and Down syndrome cell adhesion molecule like 1 (16). B-cell lymphoma protein 2 was down-regulated in HESC (21.1) and FTDC (27.1). Protein array demonstrates PIF interaction with intracellular targets insulin-degrading enzyme and beta-K+ channels. CONCLUSION: PIF displays essential multitargeted effects, of regulating immunity, promoting embryo-decidual adhesion, and regulating adaptive apoptotic processes. Copyright (c) 2010 Mosby, Inc. All rights reserved.
OBJECTIVE:Preimplantation factor (PIF) is a novel, 15 amino acid peptide, secreted by viable embryos. This study aims to elucidate PIF's effects in human endometrial stromal cells (HESC) decidualized by estrogen and progestin, which mimics the preimplantation milieu, and in first-trimester decidua cultures (FTDC). STUDY DESIGN: HESC or FTDC were incubated with 100 nmol/L synthetic PIF or vehicle control. Global gene expression was analyzed using microarray and pathway analysis. Proteins were analyzed using quantitative mass spectrometry, and PIF binding by protein array. RESULTS: Gene and proteomic analysis demonstrate that PIF affects immune, adhesion, and apoptotic pathways. Significant up-regulation in HESC (fold change) include: nuclear factor-k-beta activation via interleukin-1 receptor-associated kinase binding protein 1 (53); Toll-like receptor 5 (9); FK506 binding protein 15, 133kDa protein (2.3); and Down syndrome cell adhesion molecule like 1 (16). B-cell lymphoma protein 2 was down-regulated in HESC (21.1) and FTDC (27.1). Protein array demonstrates PIF interaction with intracellular targets insulin-degrading enzyme and beta-K+ channels. CONCLUSION:PIF displays essential multitargeted effects, of regulating immunity, promoting embryo-decidual adhesion, and regulating adaptive apoptotic processes. Copyright (c) 2010 Mosby, Inc. All rights reserved.
Authors: M Mueller; A Schoeberlein; J Zhou; M Joerger-Messerli; B Oppliger; U Reinhart; A Bordey; D Surbek; E R Barnea; Y Huang; M Paidas Journal: Cell Death Differ Date: 2015-05-15 Impact factor: 15.828
Authors: Deug-Chan Lee; Sonia S Hassan; Roberto Romero; Adi L Tarca; Gaurav Bhatti; Maria Teresa Gervasi; Joseph A Caruso; Paul M Stemmer; Chong Jai Kim; Lea Kirstine Hansen; Naja Becher; Niels Uldbjerg Journal: J Proteomics Date: 2011-03-23 Impact factor: 4.044
Authors: Christina M Duzyj; Eytan R Barnea; Min Li; S Joseph Huang; Graciela Krikun; Michael J Paidas Journal: Am J Obstet Gynecol Date: 2010-08-12 Impact factor: 8.661
Authors: Martin Mueller; Jichun Zhou; Lihua Yang; Yuan Gao; Fuju Wu; Andreina Schoeberlein; Daniel Surbek; Eytan R Barnea; Michael Paidas; Yingqun Huang Journal: Proc Natl Acad Sci U S A Date: 2014-09-09 Impact factor: 11.205
Authors: W Colin Duncan; Julie L V Shaw; Stewart Burgess; Sarah E McDonald; Hilary O D Critchley; Andrew W Horne Journal: PLoS One Date: 2011-08-17 Impact factor: 3.240