Literature DB >> 20452325

Candidate reference genes for gene expression studies in water lily.

Huolin Luo1, Sumei Chen, Hongjian Wan, Fadi Chen, Chunsun Gu, Zhaolei Liu.   

Abstract

The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder. Across all samples, clathrin adaptor complexes medium subunit (AP47) and actin 11 (ACT11) emerged as the most suitable reference genes. Across different tissues, ACT11 and elongation factor 1-alpha (EF1alpha) exhibited a stable expression pattern. ACT11 and AP47 also stably expressed in roots subjected to various treatments, but in the leaves of the same plants the most stably expressed genes were ubiquitin-conjugating enzyme 16 (UBC16) and ACT11. 2010 Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20452325     DOI： 10.1016/j.ab.2010.05.002

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

1.  Evaluation of suitable reference genes for gene expression studies in Lycoris longituba.

Authors:  Shao Jie Cui; Qiu Ling He; Ying Chen; Min Ren Huang
Journal:  J Genet       Date:  2011-12       Impact factor: 1.166

2.  Comparison of predictive methods and biological validation for qPCR reference genes in sunflower leaf senescence transcript analysis.

Authors:  Paula Fernandez; Julio A Di Rienzo; Sebastián Moschen; Guillermo A A Dosio; Luis A N Aguirrezábal; H Esteban Hopp; Norma Paniego; Ruth A Heinz
Journal:  Plant Cell Rep       Date:  2010-11-13       Impact factor: 4.570

3.  The expression of floral organ identity genes in contrasting water lily cultivars.

Authors:  Huolin Luo; Sumei Chen; Jiafu Jiang; Yu Chen; Fadi Chen; Nianjun Teng; Dongmei Yin; Changbing Huang
Journal:  Plant Cell Rep       Date:  2011-06-10       Impact factor: 4.570

4.  Selection of suitable soybean EF1α genes as internal controls for real-time PCR analyses of tissues during plant development and under stress conditions.

Authors:  Kátia D C Saraiva; Dirce Fernandes de Melo; Vanessa D Morais; Ilka M Vasconcelos; José H Costa
Journal:  Plant Cell Rep       Date:  2014-05-13       Impact factor: 4.570

5.  Selection of reference genes for quantitative real-time PCR in bamboo (Phyllostachys edulis).

Authors:  Chunjie Fan; Jinmin Ma; Qirong Guo; Xiaotie Li; Hui Wang; Mengzhu Lu
Journal:  PLoS One       Date:  2013-02-20       Impact factor: 3.240

6.  Selection of reference genes for quantitative gene expression studies in Platycladus orientalis (Cupressaceae) Using real-time PCR.

Authors:  Ermei Chang; Shengqing Shi; Jianfeng Liu; Tielong Cheng; Liang Xue; Xiuyan Yang; Wenjuan Yang; Qian Lan; Zeping Jiang
Journal:  PLoS One       Date:  2012-03-30       Impact factor: 3.240

7.  Expression stabilities of candidate reference genes for RT-qPCR under different stress conditions in soybean.

Authors:  Shuhua Ma; Hongwei Niu; Chunji Liu; Jie Zhang; Chunyan Hou; Dongmei Wang
Journal:  PLoS One       Date:  2013-10-04       Impact factor: 3.240

8.  Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development.

Authors:  Xiaojiao Han; Mengzhu Lu; Yicun Chen; Zhiyong Zhan; Qinqin Cui; Yangdong Wang
Journal:  PLoS One       Date:  2012-08-17       Impact factor: 3.240

9.  Reference gene selection in the desert plant Eremosparton songoricum.

Authors:  Xiao-Shuang Li; Hong-Lan Yang; Dao-Yuan Zhang; Yuan-Ming Zhang; Andrew J Wood
Journal:  Int J Mol Sci       Date:  2012-06-07       Impact factor: 6.208

10.  Validation of Reference Genes for Accurate Normalization of Gene Expression in Lilium davidii var. unicolor for Real Time Quantitative PCR.

Authors:  XueYan Li; JinYun Cheng; Jing Zhang; Jaime A Teixeira da Silva; ChunXia Wang; HongMei Sun
Journal:  PLoS One       Date:  2015-10-28       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.