OBJECTIVE: The aim of this study was to assess the cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and the response of rat pulp tissue to MTA and BMP-2. STUDY DESIGN: For cytotoxicity studies, 1 g MTA was mixed with or without 1 microg of BMP-2 and allowed to set for 1, 24, 48, or 72 hours before addition of samples to 2-mL aliquots of culture medium. The viability of MG-63 cells was determined using the dimethyl-thiazol-diphenyltetrazolium bromide (MTT) assay. For animal studies, upper first molars from 32 Sprague-Dawley rats were used, the molars were exposed, and 1 g MTA cement was placed in the first molars. In left molars, 1 microg BMP-2 was additionally placed on exposed pulps with MTA. After 2 weeks and 7 weeks, rats were killed and histologic sections assessed by light microscopy. RESULTS: In MTT assay, the viability was higher in the MTA with BMP-2 group than in the MTA-only group up to 24 hours, but was not significantly different thereafter. In animal study, inflammation was higher in the MTA-only group than in the MTA with BMP group, although this difference did not attain statistical significance. CONCLUSIONS: The addition of BMP-2 had a beneficial effect in vitro, reducing the initial cytotoxicity of freshly mixed MTA. However, the pulp reaction to a combination of MTA and BMP-2 was not significantly better than use of MTA alone. Copyright 2010 Mosby, Inc. All rights reserved.
OBJECTIVE: The aim of this study was to assess the cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and the response of rat pulp tissue to MTA and BMP-2. STUDY DESIGN: For cytotoxicity studies, 1 g MTA was mixed with or without 1 microg of BMP-2 and allowed to set for 1, 24, 48, or 72 hours before addition of samples to 2-mL aliquots of culture medium. The viability of MG-63 cells was determined using the dimethyl-thiazol-diphenyltetrazolium bromide (MTT) assay. For animal studies, upper first molars from 32 Sprague-Dawley rats were used, the molars were exposed, and 1 g MTA cement was placed in the first molars. In left molars, 1 microg BMP-2 was additionally placed on exposed pulps with MTA. After 2 weeks and 7 weeks, rats were killed and histologic sections assessed by light microscopy. RESULTS: In MTT assay, the viability was higher in the MTA with BMP-2 group than in the MTA-only group up to 24 hours, but was not significantly different thereafter. In animal study, inflammation was higher in the MTA-only group than in the MTA with BMP group, although this difference did not attain statistical significance. CONCLUSIONS: The addition of BMP-2 had a beneficial effect in vitro, reducing the initial cytotoxicity of freshly mixed MTA. However, the pulp reaction to a combination of MTA and BMP-2 was not significantly better than use of MTA alone. Copyright 2010 Mosby, Inc. All rights reserved.
Authors: P Chatakun; R Núñez-Toldrà; E J Díaz López; C Gil-Recio; E Martínez-Sarrà; F Hernández-Alfaro; E Ferrés-Padró; L Giner-Tarrida; M Atari Journal: Cell Mol Life Sci Date: 2013-04-09 Impact factor: 9.261
Authors: Samet Bayraktar; Pascal Jungbluth; René Deenen; Jan Grassmann; Johannes Schneppendahl; Daphne Eschbach; Armin Scholz; Joachim Windolf; Christoph V Suschek; Vera Grotheer Journal: J Tissue Eng Regen Med Date: 2017-05-19 Impact factor: 3.963