BACKGROUND: Atrial fibrillation (AF) is the most common cardiac arrhythmia found in clinical practice. We combined high-resolution two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to compare the atrial proteome of subjects with AF versus controls with sinus rhythm (SR). Our aim was to identify novel differentially regulated proteins that could be related to the development of the arrhythmia. METHODS: Human atrial appendage tissue samples from patients undergoing heart surgery with AF or SR were analyzed by high-resolution 2-DE. Proteins of interest were identified by MS and validated by western blotting and inmunohistochemistry. RESULTS: Our analysis allowed the detection of over 2300 protein spots per gel. Following differential image analysis, we found 22 spot differences between the AF and SR groups in the 4-7 isoelectric point range, leading to the identification of 15 differentially regulated proteins. The main group of proteins identified was that of heat shock proteins (HSPs), including TRAP-1, HspB3, HspΒ6 and AHA1. Some of the differences detected between AF and SR for the above proteins were due to post-translational modifications. In addition, we identified the structural protein fibulin-1 as down-regulated in atrial tissue from AF patients. CONCLUSIONS: High-resolution 2-DE analysis of human atrial tissue revealed that AF is associated with changes in structural proteins and an important number of HSPs. The lower expression of the structural protein fibulin-1 in atrial tissue from AF patients might reflect the myocardial structural changes that take place in the arrhythmia.
BACKGROUND:Atrial fibrillation (AF) is the most common cardiac arrhythmia found in clinical practice. We combined high-resolution two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to compare the atrial proteome of subjects with AF versus controls with sinus rhythm (SR). Our aim was to identify novel differentially regulated proteins that could be related to the development of the arrhythmia. METHODS:Human atrial appendage tissue samples from patients undergoing heart surgery with AF or SR were analyzed by high-resolution 2-DE. Proteins of interest were identified by MS and validated by western blotting and inmunohistochemistry. RESULTS: Our analysis allowed the detection of over 2300 protein spots per gel. Following differential image analysis, we found 22 spot differences between the AF and SR groups in the 4-7 isoelectric point range, leading to the identification of 15 differentially regulated proteins. The main group of proteins identified was that of heat shock proteins (HSPs), including TRAP-1, HspB3, HspΒ6 and AHA1. Some of the differences detected between AF and SR for the above proteins were due to post-translational modifications. In addition, we identified the structural protein fibulin-1 as down-regulated in atrial tissue from AFpatients. CONCLUSIONS: High-resolution 2-DE analysis of human atrial tissue revealed that AF is associated with changes in structural proteins and an important number of HSPs. The lower expression of the structural protein fibulin-1 in atrial tissue from AFpatients might reflect the myocardial structural changes that take place in the arrhythmia.
Authors: Rosa M Agra-Bermejo; Carla Cacho-Antonio; Adriana Rozados-Luis; Marinela Couselo-Seijas; Angel L Fernandez; J M Martinez-Cereijo; S B Bravo; Jose R Gonzalez-Juanatey; Sonia Eiras Journal: Front Physiol Date: 2020-06-30 Impact factor: 4.566
Authors: Alexandra Scholze; Else-Marie Bladbjerg; Johannes J Sidelmann; Axel C P Diederichsen; Hans Mickley; Mads Nybo; W Scott Argraves; Peter Marckmann; Lars M Rasmussen Journal: Cardiovasc Diabetol Date: 2013-01-07 Impact factor: 9.951
Authors: Esther Roselló-Lletí; Jana Alonso; Raquel Cortés; Luis Almenar; Luis Martínez-Dolz; Ignacio Sánchez-Lázaro; Francisca Lago; Inmaculada Azorín; Jose R González Juanatey; Manuel Portolés; Miguel Rivera Journal: J Cell Mol Med Date: 2012-10 Impact factor: 5.310
Authors: Jordi S Dahl; Jacob E Møller; Lars Videbæk; Mikael K Poulsen; Torsten R Rudbæk; Patricia A Pellikka; W Scott Argraves; Lars Melholt Rasmussen Journal: J Am Heart Assoc Date: 2012-12-19 Impact factor: 5.501