Literature DB >> 2044934

Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus.

M Arita1, T Honda, T Miwatani, T Takeda, T Takao, Y Shimonishi.   

Abstract

A heat-stable enterotoxin produced by Vibrio mimicus (VM-ST) was studied. VM-ST was purified from a culture supernatant of V. mimicus strain AQ-0915 by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatography on both SP-Sephadex C-50 and DEAE-Sephadex A-25, and HPLC, and the recovery rate was about 15%. Purified VM-ST was heat-stable. VM-ST activity was cross-neutralized by anti-STh antiserum. The amino acid composition of the purified VM-ST was determined 17 amino acid residues in the following sequence: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This composition and sequence were identical to those of V. cholerae non-O1-ST. These results clearly demonstrate the production of a characteristic VM-ST by V. mimicus.

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Year:  1991        PMID： 2044934     DOI： 10.1016/0378-1097(91)90536-j

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

1.  Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-O1 serogroup Hakata.

Authors:  M Arita; T Honda; T Miwatani; K Ohmori; T Takao; Y Shimonishi
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

2.  Epitope mapping and characterization of antigenic determinants of heat-stable enterotoxin (STh) of enterotoxigenic Escherichia coli by using monoclonal antibodies.

Authors:  T Takeda; G B Nair; K Suzuki; H X Zhe; Y Yokoo; P De Mol; W Hemelhof; J P Butzler; Y Takeda; Y Shimonishi
Journal:  Infect Immun       Date:  1993-01       Impact factor: 3.441

3.  Vibrio mimicus attaches to the intestinal mucosa by outer membrane hemagglutinins specific to polypeptide moieties of glycoproteins.

Authors:  M Alam; S Miyoshi; K Tomochika; S Shinoda
Journal:  Infect Immun       Date:  1997-09       Impact factor: 3.441

4.  Vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus Vibrio.

Authors:  P Yuan; A Ogawa; T Ramamurthy; G B Nair; T Shimada; S Shinoda; T Takeda
Journal:  World J Microbiol Biotechnol       Date:  1994-01       Impact factor: 3.312

5.  High prevalence of thermostable direct hemolysin (TDH)-like toxin in Vibrio mimicus strains isolated from diarrhoeal patients.

Authors:  M Uchimura; K Koiwai; Y Tsuruoka; H Tanaka
Journal:  Epidemiol Infect       Date:  1993-08       Impact factor: 2.451

Review 6.  Bacterial heat-stable enterotoxins: translation of pathogenic peptides into novel targeted diagnostics and therapeutics.

Authors:  Jieru E Lin; Michael Valentino; Glen Marszalowicz; Michael S Magee; Peng Li; Adam E Snook; Brian A Stoecker; Chang Chang; Scott A Waldman
Journal:  Toxins (Basel)       Date:  2010-08-05       Impact factor: 4.546

7.  Virulence factors in environmental and clinical Vibrio cholerae from endemic areas in Kenya.

Authors:  Racheal W Kimani; Anne W T Muigai; Willie Sang; John N Kiiru; Samuel Kariuki
Journal:  Afr J Lab Med       Date:  2014-10-17
  7 in total

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