Literature DB >> 20448544

Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry.

Xiangqing Pan1, Ruth Welti, Xuemin Wang.   

Abstract

The ability to measure plant hormones quantitatively is important as plant hormones regulate plant growth, development and response to biotic and abiotic cues. In this protocol, we describe the quantitative analysis of major plant hormones from crude plant extracts. Plant hormones are determined using reverse-phase liquid chromatography-tandem mass spectrometry with multiple reaction monitoring. The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2-3 d.

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Year:  2010        PMID： 20448544     DOI： 10.1038/nprot.2010.37

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  25 in total

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