Literature DB >> 20447418

Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein.

Hólmfrídur Sunna Gudmundsdóttir1, Katrín Olafsdóttir, Sigrídur Rut Franzdóttir, Valgerdur Andrésdóttir.   

Abstract

The construction of a molecular clone of maedi-visna virus (MVV) expressing the enhanced green fluorescent protein (EGFP) is described. The egfp gene was inserted into the gene for dUTPase since it has been shown that dUTPase is dispensable for MVV replication both in vitro and in vivo. MVV-egfp is infectious and EGFP expression is stable over at least six passages. This fluorescent virus will be a useful tool for monitoring MVV infections. Copyright 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID: 20447418     DOI: 10.1016/j.jviromet.2010.04.026

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  3 in total

1.  Propagating and detecting an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein.

Authors:  Stefán R Jónsson; Valgerdur Andrésdóttir
Journal:  J Vis Exp       Date:  2011-10-09       Impact factor: 1.355

2.  A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit.

Authors:  Chengbao Wang; Baicheng Huang; Ning Kong; Qiongyi Li; Yuping Ma; Zhijun Li; Jiming Gao; Chong Zhang; Xiangpeng Wang; Chao Liang; Lu Dang; Shuqi Xiao; Yang Mu; Qin Zhao; Yani Sun; Fernando Almazan; Luis Enjuanes; En-Min Zhou
Journal:  Vet Res       Date:  2013-10-31       Impact factor: 3.683

3.  Identification of luteolin as enterovirus 71 and coxsackievirus A16 inhibitors through reporter viruses and cell viability-based screening.

Authors:  Lin Xu; Weiheng Su; Jun Jin; Jiawen Chen; Xiaojun Li; Xuyuan Zhang; Meiyan Sun; Shiyang Sun; Peihu Fan; Dong An; Huafei Zhang; Xiguang Zhang; Wei Kong; Tonghui Ma; Chunlai Jiang
Journal:  Viruses       Date:  2014-07-17       Impact factor: 5.048

  3 in total

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