PURPOSE: To develop three-dimensional (3D) constructs of retinal pigment epithelium (RPE) and early retina progenitor cells from human embryonic stem cells (hESCs). METHODS: 3D tissue constructs were developed by culturing hESC-derived neural retinal progenitors in a matrix on top of hESC-derived RPE cells in a cell culture insert. An osmolarity gradient maintained the nutrition of the 3D cell constructs. Cross-sections through hESC-derived tissue constructs were characterized by immunohistochemistry for various transcription factors and cell markers. RESULTS: hESC-derived tissue constructs expressed transcription factors characteristic of retinal development, such as pax6, Otx2, Chx10, retinal RAX; Brn3b (necessary for differentiation of retinal ganglion cells); and crx and nrl (role in photoreceptor development). Many cells expressed neuronal markers including nestin, beta-tubulin and microtubule-associated proteins. CONCLUSIONS: This study shows for the first time that 3D early retinal progenitor tissue constructs can be derived from hESCs. Copyright 2010 Elsevier B.V. All rights reserved.
PURPOSE: To develop three-dimensional (3D) constructs of retinal pigment epithelium (RPE) and early retina progenitor cells from human embryonic stem cells (hESCs). METHODS: 3D tissue constructs were developed by culturing hESC-derived neural retinal progenitors in a matrix on top of hESC-derived RPE cells in a cell culture insert. An osmolarity gradient maintained the nutrition of the 3D cell constructs. Cross-sections through hESC-derived tissue constructs were characterized by immunohistochemistry for various transcription factors and cell markers. RESULTS: hESC-derived tissue constructs expressed transcription factors characteristic of retinal development, such as pax6, Otx2, Chx10, retinal RAX; Brn3b (necessary for differentiation of retinal ganglion cells); and crx and nrl (role in photoreceptor development). Many cells expressed neuronal markers including nestin, beta-tubulin and microtubule-associated proteins. CONCLUSIONS: This study shows for the first time that 3D early retinal progenitor tissue constructs can be derived from hESCs. Copyright 2010 Elsevier B.V. All rights reserved.
Authors: Anat Yanai; Christopher R J Laver; Aaron W Joe; Ishaq A Viringipurampeer; Xia Wang; Cheryl Y Gregory-Evans; Kevin Gregory-Evans Journal: Tissue Eng Part C Methods Date: 2013-03-15 Impact factor: 3.056
Authors: Magdalene J Seiler; Robert B Aramant; Melissa K Jones; Dave L Ferguson; Elizabeth C Bryda; Hans S Keirstead Journal: Graefes Arch Clin Exp Ophthalmol Date: 2014-05-13 Impact factor: 3.117
Authors: Natalie Alépée; Anthony Bahinski; Mardas Daneshian; Bart De Wever; Ellen Fritsche; Alan Goldberg; Jan Hansmann; Thomas Hartung; John Haycock; Helena Hogberg; Lisa Hoelting; Jens M Kelm; Suzanne Kadereit; Emily McVey; Robert Landsiedel; Marcel Leist; Marc Lübberstedt; Fozia Noor; Christian Pellevoisin; Dirk Petersohn; Uwe Pfannenbecker; Kerstin Reisinger; Tzutzuy Ramirez; Barbara Rothen-Rutishauser; Monika Schäfer-Korting; Katrin Zeilinger; Marie-Gabriele Zurich Journal: ALTEX Date: 2014-07-14 Impact factor: 6.043