OBJECTIVES: To identify proteins differentially expressed in schizophrenia patients, we collected 50 microl cerebrospinal fluid from 17 first-episode schizophrenia patients and 10 healthy controls. METHODS: Their proteins were separated by two-dimensional gel electrophoresis without using any depletion method and identified by mass spectrometry. RESULTS: Approximately 550 spots were detected, six of which had significantly different intensities in schizophrenia compared to control specimens. We were able to validate in individual samples the upregulation of apolipoprotein E, apolipoprotein A1 and prostaglandin-H2 D-isomerase by Western blot analyses and detect the downregulation of transthyretin, TGF-beta receptor type-1 and coiled-coil domain-containing protein 3 precursor. CONCLUSIONS: These findings may help to elucidate the disease mechanisms and confirm the hypothesis of disturbed cholesterol and phospholipid metabolism in schizophrenia, and thus reveal the final role players. Moreover, a grouped protein expression analysis of apolipoprotein E, apolipoprotein A-I, and prostaglandin-H2 D-isomerase in cerebrospinal fluid from patients might be a potential diagnostic tool for schizophrenia.
OBJECTIVES: To identify proteins differentially expressed in schizophreniapatients, we collected 50 microl cerebrospinal fluid from 17 first-episode schizophreniapatients and 10 healthy controls. METHODS: Their proteins were separated by two-dimensional gel electrophoresis without using any depletion method and identified by mass spectrometry. RESULTS: Approximately 550 spots were detected, six of which had significantly different intensities in schizophrenia compared to control specimens. We were able to validate in individual samples the upregulation of apolipoprotein E, apolipoprotein A1 and prostaglandin-H2 D-isomerase by Western blot analyses and detect the downregulation of transthyretin, TGF-beta receptor type-1 and coiled-coil domain-containing protein 3 precursor. CONCLUSIONS: These findings may help to elucidate the disease mechanisms and confirm the hypothesis of disturbed cholesterol and phospholipid metabolism in schizophrenia, and thus reveal the final role players. Moreover, a grouped protein expression analysis of apolipoprotein E, apolipoprotein A-I, and prostaglandin-H2 D-isomerase in cerebrospinal fluid from patients might be a potential diagnostic tool for schizophrenia.
Authors: Khaled Al Awam; Ida Sibylle Haußleiter; Ed Dudley; Rossen Donev; Martin Brüne; Georg Juckel; Johannes Thome Journal: J Neural Transm (Vienna) Date: 2014-05-01 Impact factor: 3.575
Authors: Alisa G Woods; Izabela Sokolowska; Regina Taurines; Manfred Gerlach; Edward Dudley; Johannes Thome; Costel C Darie Journal: J Cell Mol Med Date: 2012-06 Impact factor: 5.310
Authors: Christian Knöchel; Jonathan Kniep; Jason D Cooper; Michael Stäblein; Sofia Wenzler; Jan Sarlon; David Prvulovic; David E J Linden; Sabine Bahn; Pawel Stocki; Sureyya Ozcan; Gilberto Alves; Andre F Carvalho; Andreas Reif; Viola Oertel-Knöchel Journal: Eur Arch Psychiatry Clin Neurosci Date: 2016-08-22 Impact factor: 5.270