Literature DB >> 20445250

Crystallization and preliminary structural analyses of glutamate dehydrogenase from Peptoniphilus asaccharolyticus.

Tania F Oliveira1, John B Carrigan, Muaawia A Hamza, Michael A Sharkey, Paul C Engel, Amir R Khan.   

Abstract

Glutamate dehydrogenase (EC 1.4.1.2-4) from Peptoniphilus asaccharolyticus has been expressed as a selenomethionine-derivatized recombinant protein and diffraction-quality crystals have been grown that are suitable for structure determination. Preliminary structural analyses indicate that the protein assembles as a homohexameric enzyme complex in solution, similar to other bacterial and mammalian enzymes to which its sequence identity varies between 25 and 40%. The structure will provide insight into its preference for the cofactor NADH (over NADPH) by comparisons with the known structures of mammalian and bacterial enzymes.

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Year:  2010        PMID： 20445250      PMCID： PMC2864683          DOI： 10.1107/S1744309110010006

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  17 in total

1.  Large-scale domain movements and hydration structure changes in the active-site cleft of unligated glutamate dehydrogenase from Thermococcus profundus studied by cryogenic X-ray crystal structure analysis and small-angle X-ray scattering.

Authors:  M Nakasako; T Fujisawa; S Adachi; T Kudo; S Higuchi
Journal:  Biochemistry       Date:  2001-03-13       Impact factor: 3.162

2.  Refinement of macromolecular structures by the maximum-likelihood method.

Authors:  G N Murshudov; A A Vagin; E J Dodson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1997-05-01

3.  Coot: model-building tools for molecular graphics.

Authors:  Paul Emsley; Kevin Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-11-26

4.  Purification, crystallization and preliminary X-ray diffraction studies of Rab11 in complex with Rab11-FIP2.

Authors:  William N Jagoe; Sarah R Jackson; Andrew J Lindsay; Mary W McCaffrey; Amir R Khan
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-06-26

5.  Subunit assembly and active site location in the structure of glutamate dehydrogenase.

Authors:  P J Baker; K L Britton; P C Engel; G W Farrants; K S Lilley; D W Rice; T J Stillman
Journal:  Proteins       Date:  1992-01

6.  Crystal structure of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase bound to acetyl-coenzyme A reveals a novel active site architecture.

Authors:  G Sulzenbacher; L Gal; C Peneff; F Fassy; Y Bourne
Journal:  J Biol Chem       Date:  2000-12-15       Impact factor: 5.157

7.  Crystal structure of rab11 in complex with rab11 family interacting protein 2.

Authors:  William N Jagoe; Andrew J Lindsay; Randy J Read; Airlie J McCoy; Mary W McCaffrey; Amir R Khan
Journal:  Structure       Date:  2006-08       Impact factor: 5.006

8.  Structural relationship between the hexameric and tetrameric family of glutamate dehydrogenases.

Authors:  K L Britton; P J Baker; D W Rice; T J Stillman
Journal:  Eur J Biochem       Date:  1992-11-01

9.  Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis.

Authors:  John B Carrigan; Paul C Engel
Journal:  FEBS J       Date:  2007-09-10       Impact factor: 5.542

10.  Selection, expression, and nucleotide sequencing of the glutamate dehydrogenase gene of Peptostreptococcus asaccharolyticus.

Authors:  B Snedecor; H Chu; E Chen
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490
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