BACKGROUND: Studies looking at immunohistochemical (IHC) staining of Notch receptors in breast cancer and correlation with known prognostic factors are sparse. METHODS: IHC staining for nuclear, cytoplasmic, and membrane Notch-1 (N1), Notch-4 (N4), and Jagged-1 (JAG1) was performed and correlated with known prognostic factors. RESULTS: Of 48 breast cancers, 36 (67%) were invasive, mean age was 50 years (range 43-86 years), 37 (77%) were estrogen receptor (ERα) positive, and 13 (27%) node positive. There was significantly more marked N1 membranous staining in ERα-positive tumors (P < .05). On univariate analysis, cytoplasmic N1 was significantly correlated with node status and tumor grade (P < .05); both cytoplasmic and membranous N4 significantly correlated with Ki67 (P < .05); and membranous JAG1 significantly correlated with Ki67 (P < .05). On multivariate analysis, only cytoplasmic N1 significantly correlated with node status. CONCLUSION: IHC of Notch markers is feasible and correlates with known prognostic factors consistent with a biological role of Notch signaling in breast cancer progression.
BACKGROUND: Studies looking at immunohistochemical (IHC) staining of Notch receptors in breast cancer and correlation with known prognostic factors are sparse. METHODS: IHC staining for nuclear, cytoplasmic, and membrane Notch-1 (N1), Notch-4 (N4), and Jagged-1 (JAG1) was performed and correlated with known prognostic factors. RESULTS: Of 48 breast cancers, 36 (67%) were invasive, mean age was 50 years (range 43-86 years), 37 (77%) were estrogen receptor (ERα) positive, and 13 (27%) node positive. There was significantly more marked N1 membranous staining in ERα-positive tumors (P < .05). On univariate analysis, cytoplasmic N1 was significantly correlated with node status and tumor grade (P < .05); both cytoplasmic and membranous N4 significantly correlated with Ki67 (P < .05); and membranous JAG1 significantly correlated with Ki67 (P < .05). On multivariate analysis, only cytoplasmic N1 significantly correlated with node status. CONCLUSION: IHC of Notch markers is feasible and correlates with known prognostic factors consistent with a biological role of Notch signaling in breast cancer progression.
Authors: Kinnari Pandya; Debra Wyatt; Brian Gallagher; Deep Shah; Andrew Baker; Jeffrey Bloodworth; Andrei Zlobin; Antonio Pannuti; Andrew Green; Ian O Ellis; Aleksandra Filipovic; Jason Sagert; Ajay Rana; Kathy S Albain; Lucio Miele; Mitchell F Denning; Clodia Osipo Journal: Clin Cancer Res Date: 2015-09-08 Impact factor: 12.531
Authors: He Zhu; Feriyl Bhaijee; Nivin Ishaq; Dominique J Pepper; Kandis Backus; Alexandra S Brown; Xinchun Zhou; Lucio Miele Journal: Am J Cancer Res Date: 2013-04-03 Impact factor: 6.166