Literature DB >> 20441997

The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity.

Robert P de Vries1, Erik de Vries, Berend Jan Bosch, Raoul J de Groot, Peter J M Rottier, Cornelis A M de Haan.   

Abstract

In this study we evaluated the receptor-binding properties of recombinant soluble hemagglutinin (HA) trimers (subtype H2 and H7) produced in insect S2 cells, human HEK293T or HEK293S GnTI(-) cells, which produce proteins with paucimannose, complex or high-mannose N-linked glycans, respectively. The results show that HA proteins that only differ in their glycosylation status possess different receptor fine specificities. HEK293T cell-produced HA displayed a very narrow receptor specificity. However, when treated with neuraminidase this HA was able to bind more glycans with similar specificity as HEK293S GnTI(-) cell-produced HA. Insect cell-produced HA demonstrated decreased receptor specificity. As a consequence, differences in HA fine receptor specificities could not be observed with the insect cell-, but were readily detected with the HEK293S GnTI(-) cell-produced HAs. Copyright 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20441997     DOI： 10.1016/j.virol.2010.03.047

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  49 in total

1.  A general strategy for the chemoenzymatic synthesis of asymmetrically branched N-glycans.

Authors:  Zhen Wang; Zoeisha S Chinoy; Shailesh G Ambre; Wenjie Peng; Ryan McBride; Robert P de Vries; John Glushka; James C Paulson; Geert-Jan Boons
Journal:  Science       Date:  2013-07-26       Impact factor: 47.728

2.  Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6.

Authors:  Junko Morita; Kazuki Kato; Emiko Mihara; Ryuichiro Ishitani; Junichi Takagi; Hiroshi Nishimasu; Junken Aoki; Osamu Nureki
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-05-24       Impact factor: 1.056

3.  Identification of Residues That Affect Oligomerization and/or Enzymatic Activity of Influenza Virus H5N1 Neuraminidase Proteins.

Authors:  Meiling Dai; Hongbo Guo; Jos C F M Dortmans; Jojanneke Dekkers; Johan Nordholm; Robert Daniels; Frank J M van Kuppeveld; Erik de Vries; Cornelis A M de Haan
Journal:  J Virol       Date:  2016-09-29       Impact factor: 5.103

4.  Recombinant soluble, multimeric HA and NA exhibit distinctive types of protection against pandemic swine-origin 2009 A(H1N1) influenza virus infection in ferrets.

Authors:  Berend Jan Bosch; Rogier Bodewes; Robert P de Vries; Joost H C M Kreijtz; Willem Bartelink; Geert van Amerongen; Guus F Rimmelzwaan; Cornelis A M de Haan; Albert D M E Osterhaus; Peter J M Rottier
Journal:  J Virol       Date:  2010-08-04       Impact factor: 5.103

5.  Antigenic and immunogenic properties of recombinant hemagglutinin proteins from H1N1 A/Brisbane/59/07 and B/Florida/04/06 when produced in various protein expression systems.

Authors:  Felix W Santiago; Kris Lambert Emo; Theresa Fitzgerald; John J Treanor; David J Topham
Journal:  Vaccine       Date:  2012-05-15       Impact factor: 3.641

6.  Only two residues are responsible for the dramatic difference in receptor binding between swine and new pandemic H1 hemagglutinin.

Authors:  Robert P de Vries; Erik de Vries; Karen S Moore; Alan Rigter; Peter J M Rottier; Cornelis A M de Haan
Journal:  J Biol Chem       Date:  2010-12-20       Impact factor: 5.157

7.  N-Glycosylation of Seasonal Influenza Vaccine Hemagglutinins: Implication for Potency Testing and Immune Processing.

Authors:  Yanming An; Lisa M Parsons; Ewa Jankowska; Darya Melnyk; Manju Joshi; John F Cipollo
Journal:  J Virol       Date:  2019-01-04       Impact factor: 5.103

8.  Enhanced immunogenicity of stabilized trimeric soluble influenza hemagglutinin.

Authors:  William C Weldon; Bao-Zhong Wang; Maria P Martin; Dimitrios G Koutsonanos; Ioanna Skountzou; Richard W Compans
Journal:  PLoS One       Date:  2010-09-01       Impact factor: 3.240

9.  Glycan-dependent immunogenicity of recombinant soluble trimeric hemagglutinin.

Authors:  Robert P de Vries; Cornelis H Smit; Erwin de Bruin; Alan Rigter; Erik de Vries; Lisette A H M Cornelissen; Dirk Eggink; Nancy P Y Chung; John P Moore; Rogier W Sanders; Cornelis H Hokke; Marion Koopmans; Peter J M Rottier; Cornelis A M de Haan
Journal:  J Virol       Date:  2012-08-22       Impact factor: 5.103

10.  Characterization of Epitope-Specific Anti-Respiratory Syncytial Virus (Anti-RSV) Antibody Responses after Natural Infection and after Vaccination with Formalin-Inactivated RSV.

Authors:  Ivy Widjaja; Oliver Wicht; Willem Luytjes; Kees Leenhouts; Peter J M Rottier; Frank J M van Kuppeveld; Bert Jan Haijema; Cornelis A M de Haan
Journal:  J Virol       Date:  2016-06-10       Impact factor: 5.103
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