Hong Fang1, De-sheng Ning, Xiao-yan Liang. 1. Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Academia Sinica, Guilin 541006, China. GXZWSFH@yahoo.cn
Abstract
OBJECTIVE: To study the optimum extraction conditions of triterpenoid saponins from Picria felterrae. METHODS: The optimum extraction conditions were investigated by the contents of the total glycosides and picfeltarraenine I A and I B, using orthogonal test. RESULTS: The optimum extraction conditions were as follows: using 70% alcohol of 19 times than the amount of original material soaking 3 hours, extracting twice with hot reflux method at 80 degrees C, 3h the first time, 1 h the second time. CONCLUSION: The selected extraction conditions are convenient and practical with high accuracy, and could be used as a reference for industrial production.
OBJECTIVE: To study the optimum extraction conditions of triterpenoidsaponins from Picria felterrae. METHODS: The optimum extraction conditions were investigated by the contents of the total glycosides and picfeltarraenine I A and I B, using orthogonal test. RESULTS: The optimum extraction conditions were as follows: using 70% alcohol of 19 times than the amount of original material soaking 3 hours, extracting twice with hot reflux method at 80 degrees C, 3h the first time, 1 h the second time. CONCLUSION: The selected extraction conditions are convenient and practical with high accuracy, and could be used as a reference for industrial production.