Literature DB >> 20430886

Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation.

Zhe Chen1, Frank Medina, Mu-ya Liu, Celestine Thomas, Stephen R Sprang, Paul C Sternweis.   

Abstract

Guanine nucleotide exchange factors (GEFs) catalyze exchange of GDP for GTP by stabilizing the nucleotide-free state of the small GTPases through their Dbl homology/pleckstrin homology (DH.PH) domains. Unconventionally, PDZ-RhoGEF (PRG), a member of the RGS-RhoGEFs, binds tightly to both nucleotide-free and activated RhoA (RhoA.GTP). We have characterized the interaction between PRG and activated RhoA and determined the structure of the PRG-DH.PH-RhoA.GTPgammaS (guanosine 5'-O-[gamma-thio]triphosphate) complex. The interface bears striking similarity to a GTPase-effector interface and involves the switch regions in RhoA and a hydrophobic patch in PRG-PH that is conserved among all Lbc RhoGEFs. The two surfaces that bind activated and nucleotide-free RhoA on PRG-DH.PH do not overlap, and a ternary complex of PRG-DH.PH bound to both forms of RhoA can be isolated by size-exclusion chromatography. This novel interaction between activated RhoA and PH could play a key role in regulation of RhoGEF activity in vivo.

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Year:  2010        PMID： 20430886      PMCID： PMC2898337          DOI： 10.1074/jbc.M110.122549

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  43 in total

1.  The Protein Data Bank.

Authors:  H M Berman; J Westbrook; Z Feng; G Gilliland; T N Bhat; H Weissig; I N Shindyalov; P E Bourne
Journal:  Nucleic Acids Res       Date:  2000-01-01       Impact factor: 16.971

2.  Electrostatics of nanosystems: application to microtubules and the ribosome.

Authors:  N A Baker; D Sept; S Joseph; M J Holst; J A McCammon
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-21       Impact factor: 11.205

3.  Identification of potential mechanisms for regulation of p115 RhoGEF through analysis of endogenous and mutant forms of the exchange factor.

Authors:  C D Wells; S Gutowski; G Bollag; P C Sternweis
Journal:  J Biol Chem       Date:  2001-05-30       Impact factor: 5.157

Review 4.  Guanine nucleotide exchange factors for Rho GTPases: turning on the switch.

Authors:  Anja Schmidt; Alan Hall
Journal:  Genes Dev       Date:  2002-07-01       Impact factor: 11.361

5.  Modulation of the neuronal glutamate transporter EAAT4 by two interacting proteins.

Authors:  M Jackson; W Song; M Y Liu; L Jin; M Dykes-Hoberg; C I Lin; W J Bowers; H J Federoff; P C Sternweis; J D Rothstein
Journal:  Nature       Date:  2001-03-01       Impact factor: 49.962

6.  Galpha 13 requires palmitoylation for plasma membrane localization, Rho-dependent signaling, and promotion of p115-RhoGEF membrane binding.

Authors:  R Bhattacharyya; P B Wedegaertner
Journal:  J Biol Chem       Date:  2000-05-19       Impact factor: 5.157

7.  The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1.

Authors:  R Maesaki; K Ihara; T Shimizu; S Kuroda; K Kaibuchi; T Hakoshima
Journal:  Mol Cell       Date:  1999-11       Impact factor: 17.970

Review 8.  Molecular determinants in pleckstrin homology domains that allow specific recognition of phosphoinositides.

Authors:  M A Lemmon; K M Ferguson
Journal:  Biochem Soc Trans       Date:  2001-08       Impact factor: 5.407

Review 9.  The guanine nucleotide-binding switch in three dimensions.

Authors:  I R Vetter; A Wittinghofer
Journal:  Science       Date:  2001-11-09       Impact factor: 47.728

10.  Direct interaction of insulin-like growth factor-1 receptor with leukemia-associated RhoGEF.

Authors:  S Taya; N Inagaki; H Sengiku; H Makino; A Iwamatsu; I Urakawa; K Nagao; S Kataoka; K Kaibuchi
Journal:  J Cell Biol       Date:  2001-11-26       Impact factor: 10.539
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  34 in total

1.  Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain.

Authors:  Zhe Chen; Liang Guo; Jana Hadas; Stephen Gutowski; Stephen R Sprang; Paul C Sternweis
Journal:  J Biol Chem       Date:  2012-06-01       Impact factor: 5.157

2.  Plasma membrane association of p63 Rho guanine nucleotide exchange factor (p63RhoGEF) is mediated by palmitoylation and is required for basal activity in cells.

Authors:  Mohamed Aittaleb; Akiyuki Nishimura; Maurine E Linder; John J G Tesmer
Journal:  J Biol Chem       Date:  2011-08-10       Impact factor: 5.157

Review 3.  Signalling mechanisms of RhoGTPase regulation by the heterotrimeric G proteins G12 and G13.

Authors:  Tohru Kozasa; Nicole Hajicek; Christina R Chow; Nobuchika Suzuki
Journal:  J Biochem       Date:  2011-08-26       Impact factor: 3.387

4.  Structure of the Rho-specific guanine nucleotide-exchange factor Xpln.

Authors:  Kazutaka Murayama; Miyuki Kato-Murayama; Ryogo Akasaka; Takaho Terada; Shigeyuki Yokoyama; Mikako Shirouzu
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-11-19

5.  Crystal structure of mouse RhoA:GTPγS complex in a centered lattice.

Authors:  Chacko Jobichen; Kuntal Pal; Kunchithapadam Swaminathan
Journal:  J Struct Funct Genomics       Date:  2012-09-22

Review 6.  RhoGEFs in cell motility: novel links between Rgnef and focal adhesion kinase.

Authors:  N L G Miller; E G Kleinschmidt; D D Schlaepfer
Journal:  Curr Mol Med       Date:  2014-02       Impact factor: 2.222

7.  Calmodulin and PI(3,4,5)P₃ cooperatively bind to the Itk pleckstrin homology domain to promote efficient calcium signaling and IL-17A production.

Authors:  Xinxin Wang; Scott E Boyken; Jiancheng Hu; Xiaolu Xu; Ryan P Rimer; Madeline A Shea; Andrey S Shaw; Amy H Andreotti; Yina H Huang
Journal:  Sci Signal       Date:  2014-08-05       Impact factor: 8.192

8.  Prenylation and membrane localization of Cdc42 are essential for activation by DOCK7.

Authors:  Yeyun Zhou; Jared L Johnson; Richard A Cerione; Jon W Erickson
Journal:  Biochemistry       Date:  2013-06-14       Impact factor: 3.162

9.  Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission.

Authors:  Simone Mayer; Rohit Kumar; Mamta Jaiswal; Tolga Soykan; Mohammad Reza Ahmadian; Nils Brose; Heinrich Betz; Jeong-Seop Rhee; Theofilos Papadopoulos
Journal:  Proc Natl Acad Sci U S A       Date:  2013-12-02       Impact factor: 11.205

10.  The RhoGAP activity of CYK-4/MgcRacGAP functions non-canonically by promoting RhoA activation during cytokinesis.

Authors:  Donglei Zhang; Michael Glotzer
Journal:  Elife       Date:  2015-08-07       Impact factor: 8.140
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