OBJECTIVE: To investigate oxidative stress and angiogenetic factors in polycystic ovary syndrome (PCOS). DESIGN: Prospective cohort study. SETTING: University outpatient clinic. SUBJECTS: Fifty women with PCOS were divided into two groups: body mass index (BMI) >27 kg/m(2) (n = 25) and BMI <27 kg/m(2) (n = 25). The control group consisted of 20 age- and BMI-matched healthy women. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Enzyme-linked immunosorbent assays were used to measure serum levels of 8-hydroxydeoxyguanosine (8-OHdG), angiopoietin-2, and vascular endothelial growth factor (VEGF). RESULT(S): Women with PCOS had significantly lower serum 8-OHdG levels (mean 137.8 pg/mL, range 53.4-282.9 pg/mL) compared with the control subjects (mean 219.78 pg/mL, 124.6-372.4 pg/mL). This difference was obvious in both lean (BMI <27 kg/m(2)) and obese (BMI >27 kg/m(2)) subjects. Concentrations of VEGF were higher among obese subjects with PCOS. CONCLUSION(S): Serum 8-OHdG levels are significantly lower in women with PCOS than in healthy controls. The clinical significance of this finding is discussed.
OBJECTIVE: To investigate oxidative stress and angiogenetic factors in polycystic ovary syndrome (PCOS). DESIGN: Prospective cohort study. SETTING: University outpatient clinic. SUBJECTS: Fifty women with PCOS were divided into two groups: body mass index (BMI) >27 kg/m(2) (n = 25) and BMI <27 kg/m(2) (n = 25). The control group consisted of 20 age- and BMI-matched healthy women. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Enzyme-linked immunosorbent assays were used to measure serum levels of 8-hydroxydeoxyguanosine (8-OHdG), angiopoietin-2, and vascular endothelial growth factor (VEGF). RESULT(S): Women with PCOS had significantly lower serum 8-OHdG levels (mean 137.8 pg/mL, range 53.4-282.9 pg/mL) compared with the control subjects (mean 219.78 pg/mL, 124.6-372.4 pg/mL). This difference was obvious in both lean (BMI <27 kg/m(2)) and obese (BMI >27 kg/m(2)) subjects. Concentrations of VEGF were higher among obese subjects with PCOS. CONCLUSION(S): Serum 8-OHdG levels are significantly lower in women with PCOS than in healthy controls. The clinical significance of this finding is discussed.
Authors: Johanna Pölönen; Pekka Pinola; Justiina Ronkainen; Alex I Blakemore; Jessica L Buxton; Juha S Tapanainen; Stephen Franks; Terhi T Piltonen; Sylvain Sebert; Laure Morin-Papunen Journal: Eur J Endocrinol Date: 2022-09-29 Impact factor: 6.558