Identification of differential proteins in colon cancer SW480 cells with HIF1-alpha silence by proteome analysis.

Hypoxia is one of the basic characteristics of the colorectal cancer and HIF1 plays a central role in tumor hypoxia adaptation and controls the expression of a variety of genes. But it had not reached a comprehensive understanding of them in colorectal cancer in hypoxia. This study silenced HIF1-alpha, which is the regulatory subunit of HIF1, and established two dimensional gel electrophoresis profiles for human SW480siHIF1-alpha and SW480N cells to identified proteins with differential expressions. Ten down-regulated proteins and nine up-regulated proteins were identified by HPLC-Chip-MS/MS. Down-regulated proteins including PLD2, ANXA2. HIF1-alpha had a clear positive correlation with PLD2 and ANXA2 in colon adenocarcinoma samples. There proteins might have a direct or indirect contact HIF1-alpha, and play a critical role in apoptosis, signal transduction and hypoxia adaptation.