Literature DB >> 2042741

"Pocket blotting": a method for transferring nucleic acids onto nylon membranes.

G Cuny1, F Veas, G Roizès.   

Abstract

We have developed a fast and efficient method for transferring nucleic acids onto nylon membranes. This method requires less DNA for transfer; no decrease in efficiency is observed after successive probing, and several gels can be processed simultaneously. We believe that this techniques is of general interest in routine analysis of multiple samples in population genetic studies or in diagnosis purposes.

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Year:  1991        PMID： 2042741     DOI： 10.1016/0003-2697(91)90041-q

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  DNA fingerprinting in schistosome-vector snails.

Authors:  P Jarne; B Delay; C Bellec; G Roizes; G Cuny
Journal:  Biochem Genet       Date:  1990-12       Impact factor: 1.890

2.  The lipid-transfer protein C of Ricinus communis L.: isolation of two cDNA sequences which are strongly and exclusively expressed in cotyledons after germination.

Authors:  A Weig; E Komor
Journal:  Planta       Date:  1992-06       Impact factor: 4.116

3.  Protein kinase C isoenzymes in airway smooth muscle.

Authors:  B L Webb; M A Lindsay; P J Barnes; M A Giembycz
Journal:  Biochem J       Date:  1997-05-15       Impact factor: 3.857

4.  Identification of the nik gene cluster of Brucella suis: regulation and contribution to urease activity.

Authors:  V Jubier-Maurin; A Rodrigue; S Ouahrani-Bettache; M Layssac; M A Mandrand-Berthelot; S Köhler; J P Liautard
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

5.  Genetic and molecular analysis of familial isolated growth hormone deficiency.

Authors:  R Ruiz-Pacheco; P Chatelain; P C Sizonenko; M Bost; P Garandau; C Sultan
Journal:  Hum Genet       Date:  1993-10-01       Impact factor: 4.132
  5 in total

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