OBJECTIVE: To investigate the regulatory role of JAK2/STAT3/vimentin signaling pathway on the proliferation and migration of human colon cancer cells. METHODS: The human colon cancer cell Lovo was treated with Janus kinase inhibitor AG490. The proliferation of Lovo cells was quantified by MTT assay. The migration of Lovo cells was measured with scratch assay. The intracellular phosphorylation STAT3 (P-STAT3) and vimentin protein was detected by Western blot and immunofluorescence. RESULTS: After AG490 treatment, the proliferative ability of Lovo cells decreased as compared with control group (P<0.05). This suppression was dose-independent and time-independent. At 24 hrs after scratching, scratch width in the AG490 group recovered to 20%, lower than that in the control group (60%, P<0.05). After AG490 treatment, the expression of P-STAT3 and vimentin in Lovo cells decreased significantly (P<0.05). CONCLUSION: JAK2/STAT3/vimentin signaling pathway participates in regulating the proliferation and migration of human colon cancer cells.
OBJECTIVE: To investigate the regulatory role of JAK2/STAT3/vimentin signaling pathway on the proliferation and migration of humancolon cancer cells. METHODS: The humancolon cancer cell Lovo was treated with Janus kinase inhibitor AG490. The proliferation of Lovo cells was quantified by MTT assay. The migration of Lovo cells was measured with scratch assay. The intracellular phosphorylation STAT3 (P-STAT3) and vimentin protein was detected by Western blot and immunofluorescence. RESULTS: After AG490 treatment, the proliferative ability of Lovo cells decreased as compared with control group (P<0.05). This suppression was dose-independent and time-independent. At 24 hrs after scratching, scratch width in the AG490 group recovered to 20%, lower than that in the control group (60%, P<0.05). After AG490 treatment, the expression of P-STAT3 and vimentin in Lovo cells decreased significantly (P<0.05). CONCLUSION:JAK2/STAT3/vimentin signaling pathway participates in regulating the proliferation and migration of humancolon cancer cells.
Authors: Anurima Majumder; Annet Kirabo; Kanchana Karrupiah; Shigeharu Tsuda; Jennifer Caldwell-Busby; Arturo J Cardounel; György M Keseru; Peter P Sayeski Journal: Biochemistry Date: 2011-08-17 Impact factor: 3.162