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Literature DB >> 20421407

Minigenome-based reporter system suitable for high-throughput screening of compounds able to inhibit Ebolavirus replication and/or transcription.

Luke D Jasenosky¹, Gabriele Neumann, Yoshihiro Kawaoka.

Abstract

We describe an Ebolavirus minigenome-based system that is suitable for high-throughput screening of compounds able to impair Ebolavirus virus replication and/or transcription. The assay is robust (Z' factor, >0.6) and can be carried out in low-biosafety containment. Results from a pilot screen of 960 compounds are presented.

Mesh：

Substances：
Antiviral Agents

Year: 2010 PMID： 20421407 PMCID： PMC2897319 DOI： 10.1128/AAC.00138-10

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

24 in total

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Review 2. Adapting high-throughput screening methods and assays for biocontainment laboratories.

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3. An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening.

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5. Assembly of the Ebola Virus Nucleoprotein from a Chaperoned VP35 Complex.

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6. Inhibiting pyrimidine biosynthesis impairs Ebola virus replication through depletion of nucleoside pools and activation of innate immune responses.

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