Literature DB >> 20419442

Xenopus laevis Oocytes.

Stefan Bröer1.   

Abstract

Xenopus oocytes are a versatile expression system particularly suited for membrane transporters and channels. Oocytes have little background activity and therefore offer a very high signal-to-noise ratio for transporter and channel characterization. This chapter provides an overview of the basic methods used for the analysis of membrane transporters in this system, including preparation of oocytes, assays of transport activity, protocols for immunostaining and fluorescence microscopy, and other assays to study surface expression.

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Year:  2010        PMID： 20419442     DOI： 10.1007/978-1-60761-700-6_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

1.  Can one Bad Egg' really spoil the batch?

Authors:  Hanne B Moeller; Robert A Fenton
Journal:  J Physiol       Date:  2010-07-01       Impact factor: 5.182

2.  Significance of short chain fatty acid transport by members of the monocarboxylate transporter family (MCT).

Authors:  Ivano Moschen; Angelika Bröer; Sandra Galić; Florian Lang; Stefan Bröer
Journal:  Neurochem Res       Date:  2012-08-10       Impact factor: 3.996

3.  A lactate and formate transporter in the intraerythrocytic malaria parasite, Plasmodium falciparum.

Authors:  Rosa V Marchetti; Adele M Lehane; Sarah H Shafik; Markus Winterberg; Rowena E Martin; Kiaran Kirk
Journal:  Nat Commun       Date:  2015-03-31       Impact factor: 14.919

4.  Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth.

Authors:  Benjamin Weller; Melina Zourelidou; Lena Frank; Inês C R Barbosa; Astrid Fastner; Sandra Richter; Gerd Jürgens; Ulrich Z Hammes; Claus Schwechheimer
Journal:  Proc Natl Acad Sci U S A       Date:  2017-01-17       Impact factor: 11.205

5.  Identification of novel inhibitors of the amino acid transporter B0 AT1 (SLC6A19), a potential target to induce protein restriction and to treat type 2 diabetes.

Authors:  Qi Cheng; Nishank Shah; Angelika Bröer; Stephen Fairweather; Yang Jiang; Dieter Schmoll; Ben Corry; Stefan Bröer
Journal:  Br J Pharmacol       Date:  2017-02-14       Impact factor: 8.739

6.  Malaria parasite type 4 equilibrative nucleoside transporters (ENT4) are purine transporters with distinct substrate specificity.

Authors:  I J Frame; Emilio F Merino; Vern L Schramm; María B Cassera; Myles H Akabas
Journal:  Biochem J       Date:  2012-09-01       Impact factor: 3.857

7.  Diverse mutational pathways converge on saturable chloroquine transport via the malaria parasite's chloroquine resistance transporter.

Authors:  Robert L Summers; Anurag Dave; Tegan J Dolstra; Sebastiano Bellanca; Rosa V Marchetti; Megan N Nash; Sashika N Richards; Valerie Goh; Robyn L Schenk; Wilfred D Stein; Kiaran Kirk; Cecilia P Sanchez; Michael Lanzer; Rowena E Martin
Journal:  Proc Natl Acad Sci U S A       Date:  2014-04-11       Impact factor: 11.205

8.  Role of N-glycosylation in renal betaine transport.

Authors:  Eva S Schweikhard; Birgitta C Burckhardt; Friedericke Joos; Cristina Fenollar-Ferrer; Lucy R Forrest; Stephen A Kempson; Christine Ziegler
Journal:  Biochem J       Date:  2015-06-11       Impact factor: 3.857

Review 9.  Metabolism, compartmentation, transport and production of acetate in the cortical brain tissue slice.

Authors:  Caroline Rae; Aurélie D Fekete; Mohammed A Kashem; Fatima A Nasrallah; Stefan Bröer
Journal:  Neurochem Res       Date:  2012-08-01       Impact factor: 3.996

10.  Dictyostelium Nramp1, which is structurally and functionally similar to mammalian DMT1 transporter, mediates phagosomal iron efflux.

Authors:  Simona Buracco; Barbara Peracino; Raffaella Cinquetti; Elena Signoretto; Alessandra Vollero; Francesca Imperiali; Michela Castagna; Elena Bossi; Salvatore Bozzaro
Journal:  J Cell Sci       Date:  2015-07-24       Impact factor: 5.285
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