S Chandel1, U Bagai. 1. Department of Zoology, Panjab University, Chandigarh, India.
Abstract
BACKGROUND & OBJECTIVES: The present work was undertaken to evaluate antiplasmodial activity of ethanolic leaves extract of traditional medicinal plant Ajuga bracteosa in Plasmodium berghei infected BALB/c mice along with its phytochemical screening and acute toxicity test to support its traditional use as a remedy for malaria. METHODS: Plant extract (ethanolic) 250, 500, 750 mg/kg/day was evaluated in the early and established infection along with repository activity in P. berghei infected BALB/c mice through suppressive, curative and preventive test. The phytochemical screening was carried out by employing standard procedures. The acute toxicity was checked through limit test. RESULTS: The ethanolic leaves extract of A. bracteosa (250, 500 and 750 mg/kg/day) demonstrated a dose-dependent chemosuppression during early and in established infections, along with significant (P<0.05) repository activity. At a concentration of 750 mg/kg/day maximum 77.7 per cent chemosuppression during early infection and 68.8 per cent chemosuppression in repository activity were found. This dose enhanced significant mean survival period up to 27.4 +/- 0.46 days in established infection. ELEAB was found to be safe up to 5 g/kg weight when administrated orally in the female BALB/c mice, which is upper limit for oral administration of the test material to rodents. ED(50) of ELEAB was 300 mg/kg body weight of mice. INTERPRETATION & CONCLUSION: ELEAB inhibited parasitaemia and enhanced mean survival time in a dose- dependent manner upto 750 mg/kg/day dose in treated mice. Further studies need to be done to isolate and characterize active constituents of extract and to study their mechanism of action.
BACKGROUND & OBJECTIVES: The present work was undertaken to evaluate antiplasmodial activity of ethanolic leaves extract of traditional medicinal plant Ajuga bracteosa in Plasmodium berghei infected BALB/c mice along with its phytochemical screening and acute toxicity test to support its traditional use as a remedy for malaria. METHODS: Plant extract (ethanolic) 250, 500, 750 mg/kg/day was evaluated in the early and established infection along with repository activity in P. berghei infected BALB/c mice through suppressive, curative and preventive test. The phytochemical screening was carried out by employing standard procedures. The acute toxicity was checked through limit test. RESULTS: The ethanolic leaves extract of A. bracteosa (250, 500 and 750 mg/kg/day) demonstrated a dose-dependent chemosuppression during early and in established infections, along with significant (P<0.05) repository activity. At a concentration of 750 mg/kg/day maximum 77.7 per cent chemosuppression during early infection and 68.8 per cent chemosuppression in repository activity were found. This dose enhanced significant mean survival period up to 27.4 +/- 0.46 days in established infection. ELEAB was found to be safe up to 5 g/kg weight when administrated orally in the female BALB/c mice, which is upper limit for oral administration of the test material to rodents. ED(50) of ELEAB was 300 mg/kg body weight of mice. INTERPRETATION & CONCLUSION: ELEAB inhibited parasitaemia and enhanced mean survival time in a dose- dependent manner upto 750 mg/kg/day dose in treated mice. Further studies need to be done to isolate and characterize active constituents of extract and to study their mechanism of action.
Authors: Serge David Dago Attemene; Sylvain Beourou; Karim Tuo; Albert Alloh Gnondjui; Abibatou Konate; Andre Offianan Toure; Seraphin Kati-Coulibaly; Joseph Alico Djaman Journal: J Parasit Dis Date: 2017-11-10
Authors: Nawal M Al-Musayeib; Ramzi A Mothana; An Matheeussen; Paul Cos; Louis Maes Journal: BMC Complement Altern Med Date: 2012-04-20 Impact factor: 3.659