Literature DB >> 20418438

Characterization of a thermostable short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus.

Tatiana N Stekhanova1, Andrey V Mardanov, Ekaterina Y Bezsudnova, Vadim M Gumerov, Nikolai V Ravin, Konstantin G Skryabin, Vladimir O Popov.   

Abstract

Short-chain alcohol dehydrogenase, encoded by the gene Tsib_0319 from the hyperthermophilic archaeon Thermococcus sibiricus, was expressed in Escherichia coli, purified and characterized as an NADPH-dependent enantioselective oxidoreductase with broad substrate specificity. The enzyme exhibits extremely high thermophilicity, thermostability, and tolerance to organic solvents and salts.

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Year:  2010        PMID： 20418438      PMCID： PMC2893467          DOI： 10.1128/AEM.02797-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Alcohol dehydrogenase from Sulfolobus solfataricus.

Authors:  C A Raia; A Giordano; M Rossi
Journal:  Methods Enzymol       Date:  2001       Impact factor: 1.600

Review 2.  Improving enzymes by using them in organic solvents.

Authors:  A M Klibanov
Journal:  Nature       Date:  2001-01-11       Impact factor: 49.962

3.  Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  J van der Oost; W G Voorhorst; S W Kengen; A C Geerling; V Wittenhorst; Y Gueguen; W M de Vos
Journal:  Eur J Biochem       Date:  2001-05

4.  Production and characterization of a thermostable alcohol dehydrogenase that belongs to the aldo-keto reductase superfamily.

Authors:  Ronnie Machielsen; Agustinus R Uria; Servé W M Kengen; John van der Oost
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

5.  Purification and sequence analysis of a novel NADP(H)-dependent type III alcohol dehydrogenase from Thermococcus strain AN1.

Authors:  D Li; K J Stevenson
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Evidence for co-operativity in coenzyme binding to tetrameric Sulfolobus solfataricus alcohol dehydrogenase and its structural basis: fluorescence, kinetic and structural studies of the wild-type enzyme and non-co-operative N249Y mutant.

Authors:  Antonietta Giordano; Ferdinando Febbraio; Consiglia Russo; Mosè Rossi; Carlo A Raia
Journal:  Biochem J       Date:  2005-06-01       Impact factor: 3.857

8.  Cloning and over-expression in Escherichia coli of the gene encoding NADPH group III alcohol dehydrogenase from Thermococcus hydrothermalis. Characterization and comparison of the native and the recombinant enzymes.

Authors:  E Antoine; J L Rolland; J P Raffin; J Dietrich
Journal:  Eur J Biochem       Date:  1999-09

9.  Archaeal alcohol dehydrogenase active at increased temperatures and in the presence of organic solvents.

Authors:  Matthias Hess; Garabed Antranikian
Journal:  Appl Microbiol Biotechnol       Date:  2007-11-08       Impact factor: 4.813

10.  Purification and characterization of NADP-specific alcohol dehydrogenase and glutamate dehydrogenase from the hyperthermophilic archaeon Thermococcus litoralis.

Authors:  K Ma; F T Robb; M W Adams
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792
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  5 in total

1.  Characterization of a zinc-containing alcohol dehydrogenase with stereoselectivity from the hyperthermophilic archaeon Thermococcus guaymasensis.

Authors:  Xiangxian Ying; Kesen Ma
Journal:  J Bacteriol       Date:  2011-04-22       Impact factor: 3.490

2.  Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols.

Authors:  Xi Wu; Chong Zhang; Izumi Orita; Tadayuki Imanaka; Toshiaki Fukui; Xin-Hui Xing
Journal:  Appl Environ Microbiol       Date:  2013-01-25       Impact factor: 4.792

3.  Going from microbial ecology to genome data and back: studies on a haloalkaliphilic bacterium isolated from Soap Lake, Washington State.

Authors:  Melanie R Mormile
Journal:  Front Microbiol       Date:  2014-11-19       Impact factor: 5.640

4.  Decarboxylation of pyruvate to acetaldehyde for ethanol production by hyperthermophiles.

Authors:  Mohammad S Eram; Kesen Ma
Journal:  Biomolecules       Date:  2013-08-21

5.  Substrate scope of a dehydrogenase from Sphingomonas species A1 and its potential application in the synthesis of rare sugars and sugar derivatives.

Authors:  Barbara Beer; André Pick; Manuel Döring; Petra Lommes; Volker Sieber
Journal:  Microb Biotechnol       Date:  2018-04-26       Impact factor: 5.813
  5 in total

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