Amanda L Duffy 1 , Francisco J Olea-Popelka , James Eucher , Dahlia M Rice , Steven W Dow Show Affiliations »
Abstract
BACKGROUND: The chemokine monocyte chemoattractant protein-1 (MCP-1) is a primary regulator of monocyte mobilization from bone marrow, and increased concentrations of MCP-1 have been associated with sepsis and other inflammatory disorders in critically ill people. The relationship between MCP-1 and disease in dogs has not been evaluated previously. OBJECTIVE: The purpose of this study was to assess serum concentrations of MCP-1 in healthy dogs, dogs in the postoperative period, and critically ill dogs. We hypothesized that MCP-1 concentrations would be significantly increased in critically ill dogs compared with postoperative or healthy dogs. METHODS: Serum concentrations of MCP-1 were measured in 26 healthy control dogs, 35 postoperative dogs, and 26 critically ill dogs. Critically ill dogs were further subgrouped into dogs with sepsis, parvovirus gastroenteritis, immune-mediated hemolytic anemia, and severe trauma (n=26). MCP-1 concentrations were determined using a commercial canine MCP-1 ELISA. Associations between MCP-1 concentrations and disease status were evaluated statistically. RESULTS: MCP-1 concentration was significantly higher in critically ill dogs (median 578 pg/mL, range 144.7-1723 pg/mL) compared with healthy dogs (median 144 pg/mL, range 4.2-266.8 pg/mL) and postoperative dogs (median 160 pg/mL, range 12.6-560.4 pg/mL) (P<.001). All subgroups of critically ill dogs had increased MCP-1 concentrations with the highest concentrations occurring in dogs with sepsis. However, differences among the 4 subgroups were not statistically significant. CONCLUSION: Critically ill dogs had markedly increased serum concentrations of MCP-1 compared with postoperative and healthy dogs. These results indicate that surgery alone is not sufficient to increase MCP-1 concentrations; thus, measurement of MCP-1 may be useful in assessing disease severity in critically ill dogs. ©2010 American Society for Veterinary Clinical Pathology.
BACKGROUND: The chemokine monocyte chemoattractant protein-1 (MCP-1 ) is a primary regulator of monocyte mobilization from bone marrow, and increased concentrations of MCP-1 have been associated with sepsis and other inflammatory disorders in critically ill people . The relationship between MCP-1 and disease in dogs has not been evaluated previously. OBJECTIVE: The purpose of this study was to assess serum concentrations of MCP-1 in healthy dogs , dogs in the postoperative period, and critically ill dogs . We hypothesized that MCP-1 concentrations would be significantly increased in critically ill dogs compared with postoperative or healthy dogs . METHODS: Serum concentrations of MCP-1 were measured in 26 healthy control dogs , 35 postoperative dogs , and 26 critically ill dogs . Critically ill dogs were further subgrouped into dogs with sepsis , parvovirus gastroenteritis , immune-mediated hemolytic anemia , and severe trauma (n=26). MCP-1 concentrations were determined using a commercial canine MCP-1 ELISA. Associations between MCP-1 concentrations and disease status were evaluated statistically. RESULTS: MCP-1 concentration was significantly higher in critically ill dogs (median 578 pg/mL, range 144.7-1723 pg/mL) compared with healthy dogs (median 144 pg/mL, range 4.2-266.8 pg/mL) and postoperative dogs (median 160 pg/mL, range 12.6-560.4 pg/mL) (P<.001). All subgroups of critically ill dogs had increased MCP-1 concentrations with the highest concentrations occurring in dogs with sepsis . However, differences among the 4 subgroups were not statistically significant. CONCLUSION: Critically ill dogs had markedly increased serum concentrations of MCP-1 compared with postoperative and healthy dogs . These results indicate that surgery alone is not sufficient to increase MCP-1 concentrations; thus, measurement of MCP-1 may be useful in assessing disease severity in critically ill dogs . ©2010 American Society for Veterinary Clinical Pathology.
Entities: Disease
Gene
Species
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Year: 2010
PMID： 20412549 DOI： 10.1111/j.1939-165X.2010.00228.x
Source DB: PubMed Journal: Vet Clin Pathol ISSN： 0275-6382 Impact factor: 1.180