| Literature DB >> 2040818 |
E D Sprengers1, J A Hellings, H J Theunissen, P B Ten Kortenaar.
Abstract
Current anti-HIV-1 screening tests combine an excellent anti-HIV-1 sensitivity with a sensitivity of only 28-93% for anti-HIV-2 positive plasma or serum samples. The reactivity of anti-HIV-2 sera in anti-HIV-1 screening tests is based mainly on the immunological cross-reactivity of the GAG and POL proteins of HIV-1 and HIV-2. We describe here a sandwich immunoassay, in which HIV-1 viral lysate is combined with an HIV-2 ENV synthetic peptide, corresponding to the immunodominant envelope epitope, as the coating antigens on microELISA plates. This immunoassay has a sensitivity of 100% for anti-HIV-1 (128 sera tested) and 100% for anti-HIV-2 (109 sera tested). Assay specificity with fresh human donor sera was 99.9% (2256 sera tested).Entities:
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Year: 1991 PMID： 2040818 DOI： 10.1016/0022-1759(91)90353-h
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303