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Literature DB >> 20407887

1H, 13C, 15N backbone NMR assignments of the Staphylococcus aureus small multidrug-resistance pump (Smr) in a functionally active conformation.

Sébastien F Poget¹, Richard Harris, Sean M Cahill, Mark E Girvin.

Abstract

The plasmid-encoded small multidrug resistance pump from S. aureus transports a variety of quaternary ammonium and other hydrophobic compounds, enhancing the bacterial host's resistance to common hospital disinfectants. The protein folds as a homo-dimer of four transmembrane helices each, and appears to be fully functional only in lipid bilayers. Here we report the backbone resonance assignments and implied secondary structure for (2)H(13)C(15)N Smr reconstituted into lipid bicelles. Significant changes were observed between the chemical shifts of the protein in lipid bicelles compared to those in detergent micelles.

Entities: Chemical Gene Species

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Year: 2010 PMID： 20407887 PMCID： PMC2935522 DOI： 10.1007/s12104-010-9228-7

Source DB: PubMed Journal: Biomol NMR Assign ISSN： 1874-270X Impact factor: 0.746

19 in total

1. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.

Authors: A Krogh; B Larsson; G von Heijne; E L Sonnhammer
Journal: J Mol Biol Date: 2001-01-19 Impact factor: 5.469

2. Three-dimensional structure of the bacterial multidrug transporter EmrE shows it is an asymmetric homodimer.

Authors: Iban Ubarretxena-Belandia; Joyce M Baldwin; Shimon Schuldiner; Christopher G Tate
Journal: EMBO J Date: 2003-12-01 Impact factor: 11.598

3. An evaluation of detergents for NMR structural studies of membrane proteins.

Authors: Ray D Krueger-Koplin; Paul L Sorgen; Suzanne T Krueger-Koplin; Iván O Rivera-Torres; Sean M Cahill; David B Hicks; Leo Grinius; Terry A Krulwich; Mark E Girvin
Journal: J Biomol NMR Date: 2004-01 Impact factor: 2.835

4. Role of sequence bias in the topology of the multidrug transporter EmrE.

Authors: Hassane S McHaourab; Sanjay Mishra; Hanane A Koteiche; Sepan H Amadi
Journal: Biochemistry Date: 2008-07-11 Impact factor: 3.162

Review 5. EmrE, a model for studying evolution and mechanism of ion-coupled transporters.

Authors: Shimon Schuldiner
Journal: Biochim Biophys Acta Date: 2009-01-03

6. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal: J Biomol NMR Date: 1995-11 Impact factor: 2.835

7. Bacterial multidrug resistance is due to a single membrane protein which functions as a drug pump.

Authors: L L Grinius; E B Goldberg
Journal: J Biol Chem Date: 1994-11-25 Impact factor: 5.157

8. Structure and evolution of a family of genes encoding antiseptic and disinfectant resistance in Staphylococcus aureus.

Authors: T G Littlejohn; D DiBerardino; L J Messerotti; S J Spiers; R A Skurray
Journal: Gene Date: 1991-05-15 Impact factor: 3.688

9. The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data.

Authors: D S Wishart; B D Sykes
Journal: J Biomol NMR Date: 1994-03 Impact factor: 2.835

10. An emerging consensus for the structure of EmrE.

Authors: Vladimir M Korkhov; Christopher G Tate
Journal: Acta Crystallogr D Biol Crystallogr Date: 2009-01-20

7 in total

1. A structured loop modulates coupling between the substrate-binding and dimerization domains in the multidrug resistance transporter EmrE.

Authors: James R Banigan; Anindita Gayen; Min-Kyu Cho; Nathaniel J Traaseth
Journal: J Biol Chem Date: 2014-11-18 Impact factor: 5.157

Review 2. Review and phylogenetic analysis of qac genes that reduce susceptibility to quaternary ammonium compounds in Staphylococcus species.

Authors: Trudy M Wassenaar; David Ussery; Lene N Nielsen; Hanne Ingmer
Journal: Eur J Microbiol Immunol (Bp) Date: 2015-03-26

3. Reconstitution of integral membrane proteins into isotropic bicelles with improved sample stability and expanded lipid composition profile.

Authors: Emma A Morrison; Katherine A Henzler-Wildman
Journal: Biochim Biophys Acta Date: 2011-12-29

Review 7. Efflux Pump Mediated Antimicrobial Resistance by Staphylococci in Health-Related Environments: Challenges and the Quest for Inhibition.

Authors: Abolfazl Dashtbani-Roozbehani; Melissa H Brown
Journal: Antibiotics (Basel) Date: 2021-12-07