OBJECTIVE: To evaluate the potential of human umbilical cord-derived stromal cells (hUCSCs) as a human feeder for human embryonic stem cells (ESCs). DESIGN: Prospective study. SETTING: Laboratory of Molecular Genetics and Stem Cell Differentiation, Dental Research Institute, School of Dentistry, Seoul National University. INTERVENTION(S): The hUCSCs were established, and human ESCs were cultured on established hUCSCs without serum. MAIN OUTCOME MEASURE(S): Cell-surface markers, karyotyping, and teratoma formation. RESULT(S): Primary cultures of hUCSCs from individual umbilical cords were maintained by an established protocol. Human ESCs on hUCSC layers were successfully maintained in serum-free culture medium past passage 30. Compared with hESCs on mouse feeder cells, the hESCs on hUCSCs showed similar levels of pluripotency-related cell-surface markers, self-renewal capacity, and teratoma formation in immune-deficient mice. These ESCs cultured on hUCSCs had a normal karyotype, even after long-term culture. CONCLUSION(S): The hUCSCs supported self-renewal of hESCs in serum-free conditions. This culture system has the potential to facilitate the development of clinical-grade hESCs for regenerative medicine. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
OBJECTIVE: To evaluate the potential of human umbilical cord-derived stromal cells (hUCSCs) as a human feeder for human embryonic stem cells (ESCs). DESIGN: Prospective study. SETTING: Laboratory of Molecular Genetics and Stem Cell Differentiation, Dental Research Institute, School of Dentistry, Seoul National University. INTERVENTION(S): The hUCSCs were established, and human ESCs were cultured on established hUCSCs without serum. MAIN OUTCOME MEASURE(S): Cell-surface markers, karyotyping, and teratoma formation. RESULT(S): Primary cultures of hUCSCs from individual umbilical cords were maintained by an established protocol. Human ESCs on hUCSC layers were successfully maintained in serum-free culture medium past passage 30. Compared with hESCs on mouse feeder cells, the hESCs on hUCSCs showed similar levels of pluripotency-related cell-surface markers, self-renewal capacity, and teratoma formation in immune-deficient mice. These ESCs cultured on hUCSCs had a normal karyotype, even after long-term culture. CONCLUSION(S): The hUCSCs supported self-renewal of hESCs in serum-free conditions. This culture system has the potential to facilitate the development of clinical-grade hESCs for regenerative medicine. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: Danúbia Silva Dos Santos; Vanessa Carvalho Coelho de Oliveira; Karina Dutra Asensi; Leandro Vairo; Adriana Bastos Carvalho; Antonio Carlos Campos de Carvalho; Regina Coeli Dos Santos Goldenberg Journal: Cell Med Date: 2014-03-03
Authors: Rebekah A Neal; Steven M Lenz; Tiffany Wang; Daniel Abebayehu; Benjamin P C Brooks; Roy C Ogle; Edward A Botchwey Journal: Nanomater Environ Date: 2014-09-01
Authors: Vanessa Carvalho Coelho de Oliveira; Danúbia Silva Dos Santos; Leandro Vairo; Tais Hanae Kasai Brunswick; Luiz Alberto Soares Pimentel; Adriana Bastos Carvalho; Antonio Carlos Campos de Carvalho; Regina Coeli Dos Santos Goldenberg Journal: Exp Ther Med Date: 2017-03-08 Impact factor: 2.447